A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe

Genes & Development
Alla SigovaPhillip D Zamore

Abstract

The Schizosaccharomyces pombe genome encodes only one of each of the three major classes of proteins implicated in RNA silencing: Dicer (Dcr1), RNA-dependent RNA polymerase (RdRP; Rdp1), and Argonaute (Ago1). These three proteins are required for silencing at centromeres and for the initiation of transcriptionally silent heterochromatin at the mating-type locus. Here, we show that the introduction of a double-stranded RNA (dsRNA) hairpin corresponding to a green fluorescent protein (GFP) transgene triggers classical RNA interference (RNAi) in S. pombe. That is, GFP silencing triggered by dsRNA reflects a change in the steady-state concentration of GFP mRNA, but not in the rate of GFP transcription. RNAi in S. pombe requires dcr1, rdp1, and ago1, but does not require chp1, tas3, or swi6, genes required for transcriptional silencing. Thus, the RNAi machinery in S. pombe can direct both transcriptional and posttranscriptional silencing using a single Dicer, RdRP, and Argonaute protein. Our findings suggest that these three proteins fulfill a common biochemical function in distinct siRNA-directed silencing pathways.

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Cogoni, G Macino
Oct 3, 2000·Nature·N A SmithP M Waterhouse
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·M FagardH Vaucheret
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Oct 27, 2001·Science·R C Lee, V Ambros
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·K TakahashiM Yanagida
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 21, 2002·Molecular Biology of the Cell·Henrik MartensWolfgang Nellen
Apr 9, 2002·Genes & Development·Caterina CatalanottoCarlo Cogoni
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert W Williams, Gerald M Rubin
Aug 3, 2002·Genes & Development·Jason R KennerdellRichard W Carthew
Aug 3, 2002·Science·György Hutvágner, Phillip D Zamore
Aug 24, 2002·Science·Thomas A VolpeRobert A Martienssen
Sep 7, 2002·Science·Ira M HallShiv I S Grewal
Sep 17, 2002·Cell·Javier MartinezThomas Tuschl
Jul 31, 2003·Nucleic Acids Research·Mitch Raponi, Greg M Arndt
Dec 5, 2003·Current Biology : CB·Sarah C R Elgin, Shiv I S Grewal
Jan 6, 2004·Science·André VerdelDanesh Moazed
Mar 3, 2004·Molecular and Cellular Biology·Caterina CatalanottoCarlo Cogoni
Mar 17, 2004·PLoS Biology·Zhixin XieJames C Carrington
Mar 24, 2004·Cell·Yukihide TomariPhillip D Zamore

❮ Previous
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Citations

May 13, 2006·Current Genetics·Heriberto Cerutti, J Armando Casas-Mollano
Mar 1, 2006·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Peter J Horn, Craig L Peterson
Nov 21, 2013·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Bayly S Wheeler
Dec 2, 2009·Plant Molecular Biology·Carrie A WhittleJoan E Krochko
Feb 4, 2009·Cell Research·Ingela Djupedal, Karl Ekwall
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Gyorgy Hutvagner, Martin J Simard
Dec 15, 2010·Nature Structural & Molecular Biology·Katrina J WoolcockMarc Bühler
Mar 6, 2007·Nature Structural & Molecular Biology·Karl Ekwall
Aug 29, 2006·Nature Structural & Molecular Biology·Bethany A JanowskiDavid R Corey
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Concetta Lipardi, Bruce M Paterson
Jul 7, 2011·The Journal of Biological Chemistry·Xizhe Zhang, John J Rossi
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Tom Volpe, Robert A Martienssen
Aug 25, 2005·Cold Spring Harbor Symposia on Quantitative Biology·R MartienssenM Vaughn
Sep 12, 2009·Science·Ines A DrinnenbergDavid P Bartel
Jul 19, 2011·Eukaryotic Cell·Nikolay G KolevElisabetta Ullu
Mar 21, 2007·Molecular and Cellular Biology·Swati ChoudharyYi Liu
Aug 18, 2006·Molekuliarnaia biologiia·R N Kotel'nikovV A Gvozdev
Feb 29, 2008·Genome Biology·Julia Höck, Gunter Meister
Jul 1, 2005·RNA·Mikiko C SiomiHaruhiko Siomi
Jan 28, 2014·Biochemical and Biophysical Research Communications·Agata SmialowskaKarl Ekwall
Dec 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tomoyasu SugiyamaShiv I S Grewal
Nov 8, 2014·Journal of Experimental Botany·Zhiqiang XianZhengguo Li
Jan 13, 2006·Nature Chemical Biology·Bethany A JanowskiDavid R Corey
Dec 19, 2006·Nature Chemical Biology·Niraj H Tolia, Leemor Joshua-Tor
Feb 16, 2007·Nature Chemical Biology·John J Rossi
Dec 19, 2006·Nature Reviews. Genetics·Shiv I S Grewal, Songtao Jia
Jun 7, 2005·Expert Opinion on Therapeutic Targets·Xiu Feng Hu, Pei Xiang Xing
Sep 13, 2005·FEBS Letters·Yijun Qi, Gregory J Hannon
Sep 16, 2005·FEBS Letters·Alan J Herr
Oct 15, 2005·FEBS Letters·André Verdel, Danesh Moazed
Nov 12, 2010·Fungal Genetics and Biology : FG & B·Guilhem JanbonYong-Sun Bahn
Mar 5, 2013·Current Opinion in Cell Biology·Katrina J Woolcock, Marc Bühler
Jan 1, 2014·Molecular Cell·Ruby YuDanesh Moazed
Aug 30, 2008·The FEBS Journal·Sunit K SinghUtpal Bhadra

❮ Previous
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