A single bout of dynamic exercise by healthy adults enhances the generation of monocyte-derived-dendritic cells

Cellular Immunology
Emily C LaVoyRichard J Simpson

Abstract

The ex vivo generation of monocyte-derived-dendritic cells (mo-DCs) has facilitated the use of DCs in immunotherapy research. However, low blood monocyte numbers frequently limit the manufacture of sufficient numbers of mo-DCs for subsequent experimental and clinical procedures. Because exercise mobilizes monocytes to the blood, we tested if acute dynamic exercise by healthy adults would augment the generation of mo-DCs without compromising their differentiation or function. We compared mo-DC generation from before- and after-exercise blood over 8-days of culture. Function was assessed by FITC-dextran uptake and the stimulation of autologous cytomegalovirus (pp65)-specific-T-cells. Supporting the hypothesis, we found a near fourfold increase in number of mo-DCs generated after-exercise. Furthermore, relative FITC-dextran uptake, differentiation rate, and stimulation of pp65-specific-T-cells did not differ between before- and after-exercise mo-DCs. We conclude that exercise enhances the ex vivo generation of mo-DCs without compromising their function, and so may overcome some limitations associated with manufacturing these cells for immunotherapy.

References

Jul 1, 1994·The Journal of Experimental Medicine·N RomaniG Schuler
Aug 17, 1999·Current Opinion in Immunology·C Reis e SousaP Kaye
Jul 6, 2004·Immunological Reviews·William R HeathJose A Villadangos
Jan 16, 2007·Blood·Markus Michael WeckPeter Brossart
Feb 17, 2007·Clinical and Experimental Immunology·V FaivreA Haziot
May 16, 2007·Cancer Immunology, Immunotherapy : CII·Sandra TuyaertsAude Bonehill
Jan 12, 2010·European Journal of Applied Physiology·Marta Oliveira, Michael Gleeson
Sep 21, 2010·Cellular Immunology·Ondřej SuchánekPavel Kolář
Feb 24, 2012·European Journal of Clinical Investigation·Eduard ShantsilaGregory Y H Lip
Sep 29, 2012·Brain, Behavior, and Immunity·Emily C LaVoyRichard J Simpson
Feb 12, 2014·Current Opinion in Immunology·Kristen J RadfordMireille H Lahoud

❮ Previous
Next ❯

Citations

Apr 19, 2017·Exercise and Sport Sciences Reviews·Richard J SimpsonCatherine M Bollard
Dec 24, 2018·Antioxidants·Ning Hong AwJorming Goh
May 1, 2021·International Journal of Environmental Research and Public Health·Laura EsquiusNúria Lloberas
Jul 4, 2021·Journal for Immunotherapy of Cancer·Michael P GustafsonRichard J Simpson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.