A Single-Component Optogenetic System Allows Stringent Switch of Gene Expression in Yeast Cells

ACS Synthetic Biology
Xiaopei XuYi Yang

Abstract

Light is a highly attractive actuator that allows spatiotemporal control of diverse cellular activities. In this study, we developed a single-component light-switchable gene expression system for yeast cells, termed yLightOn system. The yLightOn system is independent of exogenous cofactors, and exhibits more than a 500-fold ON/OFF ratio, extremely low leakage, fast expression kinetics, and high spatial resolution. We demonstrated the usefulness of the yLightOn system in regulating cell growth and cell cycle by stringently controlling the expression of His3 and ΔN Sic1 genes, respectively. Furthermore, we engineered a bidirectional expression module that allows the simultaneous control of the expression of two genes by light. With ClpX and ClpP as the reporters, the fast, quantitative, and spatially specific degradation of ssrA-tagged protein was observed. We suggest that this single-component optogenetic system will be immensely helpful in understanding cellular gene regulatory networks and in the design of robust genetic circuits for synthetic biology.

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Citations

Dec 4, 2019·Biotechnology and Bioengineering·Jidapas My An-AdirekkunMegan N McClean
Jul 14, 2020·Biochemical Society Transactions·Denis HartmannMichael J Booth
May 18, 2020·Nature Communications·Gita Naseri, Mattheos A G Koffas
Oct 30, 2020·Yeast·David FigueroaFrancisco Salinas
Apr 28, 2021·Nature Chemical Biology·Edoardo RomanoBarbara Di Ventura
Jan 9, 2019·Biochemistry·Zihe LiuJens Nielsen
Jul 1, 2020·ACS Synthetic Biology·Peng ZhangYuan Lu
Aug 28, 2021·International Journal of Molecular Sciences·Andrés RomeroLuis F Larrondo
Dec 29, 2020·ACS Synthetic Biology·Peiling WuJifeng Yuan
Jan 8, 2022·ACS Synthetic Biology·Zhiqian WangHoujin Zhang

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