A single cysteine, Cys-64, is essential for assembly of tenascin-C hexabrachions.

The Journal of Biological Chemistry
J A LuczakJ E Schwarzbauer

Abstract

Tenascin-C is a large, multimeric extracellular matrix protein that is found in a variety of tissues and can have profound effects on cell adhesion. It is secreted from cells as a hexamer of six identical chains called a hexabrachion. Disulfide bonding among tenascin subunits mediates intracellular assembly into hexamers. The amino-terminal assembly domain consists of heptad repeats and at least six cysteine residues (Cys-64, -111, -113, -140, -146, -147) that could be involved in multimerization. We have now determined the requirements for these cysteine residues during hexamer assembly. Our results show that only Cys-64 is required to form the hexameric structure. Mutation of Cys-64 to glycine resulted in release of trimer intermediates, which probably form via the heptad repeats, but no hexamers were secreted. In contrast, individual or pairs of mutations of each of the other cysteines had no effect on tenascin hexamer formation, and inclusion of any other cysteine mutations along with C64G did not further disrupt the multimer pattern. However, when all six cysteines were mutated, monomers were the major extracellular form. Together, these results show that trimers are an intermediate of tenascin-C assembly and that Cys-64 i...Continue Reading

References

Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J R GulcherK Stefansson
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·F S JonesG M Edelman
Oct 1, 1989·The Journal of Cell Biology·P PeshevaM Schachner
Jan 1, 1989·Annual Review of Cell Biology·H P Erickson, M A Bourdon
Dec 1, 1989·The Journal of Cell Biology·J E SchwarzbauerC L Wilson
Jan 1, 1986·Critical Reviews in Oncology/hematology·A Z Budzynski
Dec 1, 1995·The Journal of Biological Chemistry·C Y ChungH P Erickson
Apr 1, 1994·The Journal of Cell Biology·K MatsumotoR Chiquet-Ehrismann
Sep 29, 1995·Experientia·R Chiquet-Ehrismann
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·B BergerP S Kim
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A L PrietoK L Crossin
Sep 1, 1994·Nature·P B HarburyT Alber
Jan 29, 1993·Biochemical and Biophysical Research Communications·S HuangE W Davie
Nov 22, 1996·The Journal of Biological Chemistry·J Z Zhang, C Redman

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Citations

Jul 24, 2002·Neuroscience Research·Ken-ichiro KuboKazunori Nakajima
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·P L Jones, F S Jones
Oct 22, 2002·Molecular Biology of the Cell·Kim S Midwood, Jean E Schwarzbauer
Aug 23, 2000·The Journal of Cell Biology·M B WenkJ E Schwarzbauer
Jan 24, 2012·Matrix Biology : Journal of the International Society for Matrix Biology·Deane F Mosher, Josephine C Adams
Dec 9, 2014·Cell Adhesion & Migration·Sean P Giblin, Kim S Midwood
Mar 21, 2015·Cell Adhesion & Migration·Ulrich ValcourtLaurent Bartholin
Feb 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D D Sta IglesiaM A Stepp
Nov 3, 2006·FEBS Letters·Claire LethiasJean-Yves Exposito

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