PMID: 6975760Nov 15, 1981Paper

A single-dose protocol for azaserine initiation of pancreatic carcinogenesis in the rat

International Journal of Cancer. Journal International Du Cancer
J D YagerS A Wilpone

Abstract

Previously, the induction of pancreatic carcinogenesis in the rat using azaserine has involved a multiple-dose treatment protocol. The objective of the present study was to determine the effect of multiple azaserine treatments on pancreatic DNA synthesis and to develop a protocol for a single-dose initiation of pancreatic carcinogenesis by azaserine in the rat. Pancreatic DNA synthesis in young rats, which was determined by measuring the amount of [3H]-thymidine incorporation into DNA, was found to be elevated at 4.3 weeks of age and to decrease to a baseline level by 6.3 weeks. Treatment of 4-week-old rats with azaserine resulted in a dose-dependent inhibition of [3H]-thymidine incorporation into both pancreatic and liver DNA. Maximum inhibition was seen at 10 mg/kg body weight. This inhibition was followed by a gradual return of incorporation to normal values over a 48 h period. One week following pretreatment with four weekly injections of azaserine at 30 mg/kg, [3H]-thymidine incorporation into pancreatic and liver DNA was significantly elevated, suggesting that multiple injection protocols caused enhanced DNA synthesis which could have a co-carcinogenic and/or promotional effect. Single-doses of azaserine (10, 30 and 60 mg...Continue Reading

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Citations

Jan 1, 1987·Cancer Metastasis Reviews·M S Rao
Jun 1, 1990·Digestive Diseases and Sciences·J P SmithS Bagheri
Sep 1, 1991·Cancer Causes & Control : CCC·G W OlsenL M Schuman
Nov 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D S LongneckerT J Curphey
Dec 1, 1993·Mutation Research·M W ChouR W Hart
Jun 1, 1986·Toxicology Letters·P B Krishnamurthy, G S Neelaram
Jan 1, 1985·Critical Reviews in Toxicology·T Chiu
Oct 5, 2007·Ageing Research Reviews·Mark P Mattson
Jun 1, 1984·Environmental Health Perspectives·E E McGuinnessK G Wormsley

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