PMID: 9539716May 16, 1998Paper

A single-headed dimer of Escherichia coli ribosomal protein L7/L12 supports protein synthesis

Proceedings of the National Academy of Sciences of the United States of America
A V OleinikovR R Traut

Abstract

During protein synthesis, the two elongation factors Tu and G alternately bind to the 50S ribosomal subunit at a site of which the protein L7/L12 is an essential component. L7/L12 is present in each 50S subunit in four copies organized as two dimers. Each dimer consists of distinct domains: a single N-terminal ("tail") domain that is responsible for both dimerization and binding to the ribosome via interaction with the protein L10 and two independent globular C-terminal domains ("heads") that are required for binding of elongation factors to ribosomes. The two heads are connected by flexible hinge sequences to the N-terminal domain. Important questions concerning the mechanism by which L7/L12 interacts with elongation factors are posed by us in response to the presence of two dimers, two heads per dimer, and their dynamic, mobile properties. In an attempt to answer these questions, we constructed a single-headed dimer of L7/L12 by using recombinant DNA techniques and chemical cross-linking. This chimeric molecule was added to inactive core particles lacking wild-type L7/L12 and shown to restore activity to a level approaching that of wild-type two-headed L7/L12.

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Citations

May 24, 2012·Nature Reviews. Molecular Cell Biology·Shifeng Xue, Maria Barna
Jul 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·John ChristodoulouChristopher M Dobson
Jun 14, 2005·Biophysical Journal·Joanna TrylskaJ Andrew McCammon
Nov 12, 2013·Biochemical and Biophysical Research Communications·YongJoong KimJoon Kim
Jul 27, 2004·Biopolymers·Joanna TrylskaJ Andrew McCammon
Jun 14, 2017·Molecular and Cellular Biology·Leszek WawiórkaMarek Tchórzewski
Aug 12, 2005·Journal of Molecular Recognition : JMR·Vladimir N UverskyA Keith Dunker
Nov 1, 2005·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson, Knud H Nierhaus
Sep 21, 2019·Scientific Reports·Xiaolin WangJohn Christodoulou
Feb 13, 2004·The Journal of Biological Chemistry·Eduard V BocharovAlexander S Arseniev
Mar 7, 2017·Biochemistry. Biokhimii︠a︡·I V MitroshinA G Gabdulkhakov
Jan 1, 2018·Quarterly Reviews of Biophysics·Anders Liljas, Suparna Sanyal

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