Abstract
The first extracellular domain of the alpha-subunit of the Na+/K+-ATPase (sodium/potassium pump) is functionally important, affecting sensitivity of the enzyme to cardiac glycosides (e.g. ouabain) and being implicated in the transport of K+. This domain is also variable among mammalian isoforms of the alpha-subunit. Using PCR, we have isolated from seven insect species with contrasting physiologies a DNA fragment containing this region, in order to help determine whether tissue-specific expression might be associated with isoforms encoded by a gene family, as it is in mammals. A single sequence (with one ORF) characteristic of Na+/K+-ATPase was obtained from genomic DNA of each species. Only the fragment from Manduca sexta contained an intron, but at a location different to that found in mammals. For all Diptera so far characterized, the species phylogeny is the same as the alpha-subunit gene phylogeny (based on the sequences of the first extracellular domain and flanking transmembrane domains). The results strongly indicate a single, ouabain-sensitive isoform of the alpha-subunit of Na+/K+-ATPase is present in Diptera.
References
Dec 21, 1992·FEBS Letters·F HolzingerM Wink
Apr 29, 1992·Biochimica Et Biophysica Acta·J EhrenfeldB J Harvey
Jul 22, 1991·FEBS Letters·K Geering
Sep 23, 1991·FEBS Letters·P Burgener-KairuzB C Rossier
Sep 15, 1991·Gene·M T MacíasL Sastre
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A M BertorelloP Greengard
Jan 1, 1990·The Journal of Cell Biology·S GloorM Schachner
Oct 15, 1991·The Biochemical Journal·G SchmalzingW Schwarz
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L ZhengF C Kafatos
Sep 1, 1990·Molecular Plant-microbe Interactions : MPMI·A LewinW J Broughton
Jan 1, 1990·Progress in Nucleic Acid Research and Molecular Biology·J B LingrelE M Price
May 9, 1989·Biochimica Et Biophysica Acta·K J Sweadner
Oct 23, 1989·FEBS Letters·N E BroudeE D Sverdlov
Oct 23, 1989·FEBS Letters·L A Baxter-LoweL E Hokin
Nov 1, 1988·Biochemistry·E M Price, J B Lingrel
Aug 22, 1985·Nature·G E ShullJ B Lingrel
Aug 22, 1985·Nature·K KawakamiS Inayama
Dec 16, 1986·Biochemistry·G E ShullJ B Lingrel
Dec 15, 1988·Gene·D G Higgins, P M Sharp
Feb 18, 1982·Nature·E S CoenG Dover
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Mar 1, 1994·Transactions of the Royal Society of Tropical Medicine and Hygiene·P F Billingsley
Dec 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A A al-Robai
Jul 22, 1993·Nature·A J Roger, W F Doolittle
Oct 1, 1993·Insect Biochemistry and Molecular Biology·S A Reeves, M K Yamanaka
Jan 1, 1993·Ophthalmic Research·M E SzaboP Braquet
Citations
Oct 1, 1996·Journal of Chemical Ecology·F Holzinger, M Wink
Oct 10, 2001·Genome Research·F C SerlucaM C Fishman
Aug 15, 1998·Biochemical and Biophysical Research Communications·Y ShimaH Ohta
Apr 5, 2002·Journal of Medical Entomology·A Paulo G Almeida, Peter F Billingsley
May 19, 2010·Biophysics·Tomoki KitagawaSeido Nagano
Sep 2, 1999·British Journal of Cancer·J SchneiderH J Woitowitz
Jun 15, 2006·The Journal of Membrane Biology·D Z Z HeJ Santos-Sacchi
Aug 11, 2011·PloS One·Cedric E GinestetAndrew Simmons
Apr 13, 2019·Insects·Rif S El-MallakhRajashekar Reddy Yeruva
May 29, 2003·Journal of Insect Physiology·M B.A. DjamgozA M. Emery