A Single Monoclonal Antibody against the Peanut Allergen Ara h 2 Protects against Systemic and Local Peanut Allergy

International Archives of Allergy and Immunology
Federico StorniMartin F Bachmann

Abstract

Peanut allergy is the most prevalent and dangerous food allergy. Peanuts consist of a large number of different allergens and peanut-allergic patients are frequently sensitized to multiple allergens. Hence, conventional desensitization approaches aim at targeting as many allergens as possible. The monoclonal anti-Ara h 2 antibody (mAb) was produced by hybridoma cells derived from WT BALB/c mice after immunization with a vaccine based on virus-like particles coupled to Ara h 2. BALB/c mice were sensitized intraperitoneally with peanut extract absorbed to alum and mAbs were applied i.v. Challenge was performed the next day with the whole peanut extract intravenously and via skin prick test. Here we show in peanut-allergic mice that a single high-affinity mAb specific for Ara h 2 is able to block systemic and local allergic reactions induced by the complex peanut extract. We confirm in vitro binding of the mAb to the inhibitory low-affinity FcγRIIb receptor using a sensitive biosensor and demonstrate in vivo that protection was dependent on FcγRIIb. A single mAb specific for Ara h 2 is able to improve local and systemic allergic symptoms induced by the whole allergen mixture.

References

Jul 9, 2003·The Journal of Allergy and Clinical Immunology·David M FleischerRobert A Wood
Jul 14, 2010·The Journal of Allergy and Clinical Immunology·Christina UermösiMartin F Bachmann
Jan 17, 2012·The Journal of Allergy and Clinical Immunology·Moisés A CalderónPascal Demoly
Nov 12, 2013·Allergy·B I NwaruUNKNOWN EAACI Food Allergy and Anaphylaxis Guidelines Group
Dec 21, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Livia BöhmChristian Taube
Sep 30, 2016·Therapeutic Advances in Respiratory Disease·Jasper H KappenMohamed H Shamji
Sep 25, 2017·The Journal of Allergy and Clinical Immunology·Margot BeringsCezmi A Akdis
Mar 30, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Deborah S LeuthardPål Johansen
Apr 24, 2018·Molecular Immunology·Chiara Palladino, Heimo Breiteneder
May 12, 2018·Biosensors & Bioelectronics·Gustavo Cabral-MirandaMartin F Bachmann

❮ Previous
Next ❯

Citations

Nov 17, 2020·Allergy, Asthma & Immunology Research·Phornsiri PechsrichuangAlain Jacquet
Aug 31, 2021·Frontiers in Immunology·Chiara Tontini, Silvia Bulfone-Paus

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

© 2021 Meta ULC. All rights reserved