A single mouse glutathione synthetase gene encodes six mRNAs with different 5' ends

Archives of Biochemistry and Biophysics
Z Z ShiM W Lieberman

Abstract

To understand more about the role of glutathione (GSH) in metabolism, we have cloned both cDNA and genomic sequences for mouse glutathione synthetase (GSH syn), the enzyme that catalyzes the last step in the synthesis of glutathione. The mouse cDNA contains an open reading frame (ORF) of 474 aa and shares 64 and 95% deduced amino acid sequence identity with Xenopus cDNA and rat cDNA, respectively. The cDNA complements Schizosaccaromyces pombe strains deficient in GSH syn. The gene is a single-copy gene spanning approximately 30 kb and is composed of at least 15 exons. Steady-state RNA levels and enzyme activity levels are highest in kidney, about 3-fold lower in liver, and 8- to 10-fold lower in lung and brain. We have identified six different GSH syn RNAs: three, termed types A1, A2, and A3, have different 5' ends that localize to different sites in the gene, but appear to encode the same protein (474 aa). Types B, C1, and C2 all have unique 5' ends and type-specific ORFs, which are shorter than that for types A1, A2, and A3. In liver only type A1 GSH syn RNA is detectable, while in kidney 90% of GSH syn RNA is type A1 and types B and C account for about 10%.

Citations

Nov 1, 1996·Nature Genetics·Z Z ShiM W Lieberman
Aug 8, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Krisztina DugmonitsEdit Hermesz
Aug 2, 2011·Biochemical and Biophysical Research Communications·Andreas WinklerSvante Norgren
Jul 29, 1998·Chemico-biological Interactions·L WhitbreadP G Board
Sep 29, 2015·Journal of Animal Physiology and Animal Nutrition·A BócsaiK Balogh
May 1, 2001·Current Protocols in Toxicology·C C WhiteT J Kavanagh
Aug 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G M HabibM W Lieberman
Mar 11, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·J Wernerman, F Hammarqvist
Aug 30, 2020·Fish Physiology and Biochemistry·Uğur GüllerÖmer İrfan Küfrevioğlu

Related Concepts

Mini-Exon
Cistron
Cis-Trans Test
Glutathione Synthase
Poly(A) Tail
Schizosaccharomyces pombe
Drug or Chemical Tissue Distribution
Xenopus laevis
Restriction Mapping
Gene Expression

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