PMID: 1532254Mar 15, 1992

A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis

Proceedings of the National Academy of Sciences of the United States of America
K LidholtJeffrey D Esko

Abstract

Mutants of Chinese hamster ovary cells have been found that no longer produce heparan sulfate. Characterization of one of the mutants, pgsD-677, showed that it lacks both N-acetylglucosaminyl- and glucuronosyltransferase, enzymes required for the polymerization of heparan sulfate chains. pgsD-677 also accumulates 3- to 4-fold more chondroitin sulfate than the wild type. Cell hybrids derived from pgsD-677 and wild type regained both transferase activities and the capacity to synthesize heparan sulfate. Two segregants from one of the hybrids reexpressed the dual enzyme deficiency, the lack of heparan sulfate synthesis, and the enhanced accumulation of chondroitin sulfate, suggesting that all of the traits were genetically linked. These findings indicate that the pgsD locus may represent a gene involved in the coordinate control of glycosaminoglycan formation.

References

Feb 1, 1995·Journal of Cellular Biochemistry·H Q MiaoI Vlodavsky
Dec 27, 2005·Medical Microbiology and Immunology·Donavon J HessCarol L Wells
Dec 20, 1995·Biochimica Et Biophysica Acta·J E Silbert, G Sugumaran
Nov 6, 2002·Biochimica Et Biophysica Acta·Beverly M ZakJeffrey D Esko
Sep 9, 2000·Current Opinion in Cell Biology·G H Baeg, N Perrimon
Aug 26, 1998·Trends in Microbiology·S G Andersson, C G Kurland
Mar 3, 2004·Biochemistry·Stephen M Fuchs, Ronald T Raines
Nov 11, 2010·Biochemistry·Tzu-Yuan ChaoRonald T Raines
Jul 14, 2009·Journal of the American Chemical Society·Jeffrey G MartinRobert J Linhardt
May 6, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Stéphane SarrazinJeffrey D Esko
Mar 22, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Md Nasimuzzaman, Derek A Persons
Feb 20, 1999·The Biochemical Journal·M BeltingL A Fransson
Jan 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tarja KinnunenJ E Turnbull
Aug 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Fumitoshi IrieYu Yamaguchi
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Brandon B HolmesMarc I Diamond
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A L De AgostiniR D Rosenberg
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R I MontgomeryJeffrey D Esko
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Varki
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·D A BulikS B Selleck
Sep 1, 2009·The Journal of Biological Chemistry·Zhenzhong MaJeffrey A Loeb
Oct 13, 2012·The Journal of Biological Chemistry·Kevin T BushSanjay K Nigam
May 10, 2008·The Journal of Biological Chemistry·S M Khorshed AlamMichael J Soares
Mar 1, 1992·The Journal of Cell Biology·M T ShiehP G Spear
Dec 1, 1993·The Journal of Cell Biology·R Bar-ShavitI Vlodavsky
Aug 1, 1995·The Journal of Experimental Medicine·T ChenJ Swanson
Oct 16, 2004·Molecular Biology of the Cell·Kim S MidwoodJean E Schwarzbauer
Jan 8, 2010·Glycobiology·Elina SandwallJin-Ping Li
Mar 28, 2008·Journal of Biochemistry·Satomi Nadanaka, Hiroshi Kitagawa

Citations

Sep 7, 1976·Biochemistry·J E Shively, H E Conrad
Jan 1, 1991·Annual Review of Biochemistry·L Kjellén, Ulf Lindahl
Oct 1, 1991·Current Opinion in Cell Biology·Jeffrey D Esko
Aug 26, 1988·Science·Jeffrey D EskoJ L Weinke
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey D EskoW H Taylor
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·R G HAM

Related Concepts

Ovarian Diseases
Chinese Hamster
Sulfates, Inorganic
Glycosaminoglycans
Enzymes, antithrombotic
Both Ovaries
Glucuronosyltransferase
Ovary
N-Acetylglucosaminyltransferases
Chinese Hamster Ovary Cell

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