A single mutation in the IF3 N-terminal domain perturbs the fidelity of translation initiation at three levels

Journal of Molecular Biology
Dianna MaarR W Simons

Abstract

Bacterial translation initiation factor 3 (IF3) is involved in the fidelity of translation initiation at several levels, including start-codon discrimination, mRNA translation, and initiator-tRNA selection. The IF3 C-terminal domain (CTD) is required for binding to the 30S ribosomal subunit. N-terminal domain (NTD) function is less certain, but likely contributes to initiation fidelity. Point mutations in either domain can decrease initiation fidelity, but C-terminal domain mutations may be indirect. Here, the Y75N substitution mutation in the NTD is examined in vitro and in vivo. IF3(Y75N) protein binds 30S subunits normally, but is defective in start-codon discrimination, inhibition of initiation on leaderless mRNA, and initiator-tRNA selection, thereby establishing a direct role for the IF3 NTD in these initiation processes. A model illustrating how IF3 modulates an inherent function of the 30S subunit is discussed.

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Citations

Apr 16, 2013·Nature Structural & Molecular Biology·Margaret M Elvekrog, Ruben L Gonzalez
Oct 14, 2014·Journal of Molecular Biology·Daniel D MacDougall, Ruben L Gonzalez
Oct 24, 2009·Current Opinion in Biotechnology·Taku Uchiyama, Kentaro Miyazaki
Mar 5, 2013·The Plant Journal : for Cell and Molecular Biology·Yun ShenBao-Cai Tan
Apr 21, 2012·Critical Reviews in Biochemistry and Molecular Biology·Pohl Milón, Marina V Rodnina
Nov 16, 2019·Nucleic Acids Research·Indra Mani Sharma, Sarah A Woodson
Jun 12, 2020·Nature Communications·Anas KhawajaJoanna Rorbach

Related Concepts

Alleles
Alkalescens-Dispar Group
Cis-Trans Test
Plasma Protein Binding Capacity
Ribosomes
RNA, Transfer, Initiator
Protein Biosynthesis
Tertiary Protein Structure
5' Untranslated Regions
Prokaryotic Initiation Factor-3

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