A single mutation in the Japanese encephalitis virus E protein (S123R) increases its growth rate in mouse neuroblastoma cells and its pathogenicity in mice

Virology
Shigeru TajimaI Kurane

Abstract

We previously reported that the Japanese encephalitis virus (JEV) strain Mie/41/2002 has weak pathogenicity compared with the laboratory strain Beijing-1. To identify the determinants of its growth nature and pathogenicity, we produced intertypic viruses, rJEV(EB1-M41), rJEV(nEB1-M41) and rJEV(cEB1-M41), which contained the entire, the N-terminal, and the C-terminal half, respectively, of the Beijing-1 E region in the Mie/41/2002 background. The growth of rJEV(EB1-M41) in mouse neuroblastoma N18 cells and virulence in mice were similar to those of Beijing-1. rJEV(nEB1-M41) propagated in N18 cells to the same extent as did Beijing-1. Furthermore, we produced mutant viruses with single amino acid substitutions in the N-terminal half of the Mie/41/2002 E region. A Ser-123-Arg mutation in the Mie/41/2002 E protein exhibited significantly increased growth rate in N18 cells and virulence in mice. These results indicate that the position 123 in the E protein is responsible for determining the growth properties and pathogenicity of JEV.

References

Oct 30, 2001·Virus Research·V P MishinV F Yamshchikov
Feb 14, 2003·Journal of Virology·Tom SolomonAlan D T Barrett
May 14, 2003·Journal of Virology·Sang-Im YunYoung-Min Lee
Feb 26, 2005·Journal of Virology·Yoshio MoriYoshiharu Matsuura
Sep 22, 2006·Journal of Virology·Grant E NybakkenDaved H Fremont
Feb 28, 2007·The Journal of General Virology·Huan Yu WangGuo Dong Liang
Nov 4, 2008·Emerging Infectious Diseases·Narong NitatpattanaJean-Paul Gonzalez
Jun 23, 2009·Archives of Virology·Jiu-Song ZhangWu-Chun Cao

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Citations

Jan 30, 2013·The American Journal of Tropical Medicine and Hygiene·Ryusei KuwataMasahiro Takagi
Sep 27, 2013·PLoS Neglected Tropical Diseases·Guillaume Le FlohicJean-Paul Gonzalez
Jan 15, 2011·The Journal of Veterinary Medical Science·Masayuki ShimojimaKen Maeda
Mar 13, 2015·In Vitro Cellular & Developmental Biology. Animal·Keita HoshinoToshinori Sasaki
May 14, 2016·Japanese Journal of Infectious Diseases·Toshinori SasakiMutsuo Kobayashi
Dec 28, 2017·The American Journal of Tropical Medicine and Hygiene·Darci R SmithFarooq Nasar
Aug 28, 2020·PLoS Neglected Tropical Diseases·Xuan DengHua-Kun Lv

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