PMID: 9539979Apr 16, 1998Paper

A single N-2-acetylaminofluorene adduct alters the footprint of T7 (exo-) DNA polymerase bound to a model primer-template junction

Mutation Research
D Y Burnouf, R P Fuchs

Abstract

Bovine pancreatic deoxyribonuclease I (DNaseI) has been used to footprint T7 (exo-) DNA polymerase bound to a model primer-template junction. The polymerase was blocked at a specific position either by the omission of dCTP from the reaction mix or by the presence of a N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dGuo-AAF) adduct. This lesion has been shown to be a severe block for several DNA polymerases, both in in vitro primer elongation experiments, and during the in vivo replication of AAF-monomodified single-stranded vectors. The footprints obtained with unmodified primer-template DNA define two protected domains separated by an inter-region that remains sensitive to DNaseI, and several hypersensitive sites located on both strands. Binding of the polymerase to AAF monomodified duplexes results in the same protection pattern as that obtained with the unmodified duplexes. However, the hypersensitive sites either disappear or are dramatically reduced. The results suggest that the AAF lesion alters the correct positioning of the duplex DNA within the polymerase cleft.

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I B LambertR P Fuchs
May 1, 1990·Protein Engineering·M DelarueP Argos
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D BurnoufR P Fuchs
Jun 25, 1985·Journal of Molecular Biology·M L KopkaR E Dickerson
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S K BanerjeeJ E LeClerc
Jul 1, 1984·Journal of Biochemical and Biophysical Methods·J GangloffG Dirheimer
Oct 16, 1980·Biochemical and Biophysical Research Communications·M LengP Rio
Jul 15, 1981·Journal of Molecular Biology·G P LomonossoffA Klug
Jun 1, 1984·Cell·H R Drew, A A Travers
Mar 1, 1983·Environmental Health Perspectives·J A Miller, E C Miller
May 1, 1982·Chemico-biological Interactions·K B LipkowitzF A Beland
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W MetzgerH Heumann
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·A Jacobo-MolinaP Clark
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·D C ThomasR P Fuchs
Oct 1, 1993·Current Opinion in Genetics & Development·S Murli, G C Walker
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·N Koffel-SchwartzR P Fuchs
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·J S HoffmannG Villani
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·R L NapolitanoR P Fuchs

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