A single nucleotide substitution converts potato spindle tuber viroid (PSTVd) from a noninfectious to an infectious RNA for nicotiana tabacum

Virology
M WasseneggerH L Sänger

Abstract

Mechanical inoculation of Nicotiana tabacum with the PSTVd isolate KF 440-2 from the host plant tomato resulted in the de novo emergence, replication, and accumulation of a new "tobacco variant," designated PSTVd NT. It produces no symptoms in tobacco but, like PSTV KF 440-2, severe ones in tomato. The sequence analysis of PSTVd NT revealed a single nucleotide substitution from C-->U at position 259. Autonomous viroid replication was also induced in tobacco by genomic integration of oligomeric cDNA copies of PSTVd KF 440-2. Although these cDNAs contained the original tomato-specific C259, the circular PSTVd RNA subsequently accumulating in tobacco also exhibited the C259-->U259 substitution. In the secondary structure of PSTVd, nucleotide 259 is part of an internal loop analogous to loop E of eukaryotic 5S rRNA and presumed to be the only bulged extrahelical nucleotide of this loop. The C259 in PSTVd KF 440-2 and in practically all other isolates and the U259 in PSTVd NT of the loop E-like structure might be involved in protein binding and in viroid processing. The new variant PSTVd NT is genetically stable in both tobacco and tomato.

Citations

Jan 26, 2007·Molecular Plant-microbe Interactions : MPMI·Biao Ding, Asuka Itaya
Dec 6, 2008·Phytopathology·Yosuke Matsushita, Kumar K R Penmetcha
Aug 31, 2006·Journal of Virology·Carmen HernándezRicardo Flores
Aug 5, 2005·Annual Review of Phytopathology·Ricardo FloresFrancesco Di Serio
Dec 7, 2007·PLoS Pathogens·María-Eugenia GasJosé-Antonio Daròs
Apr 15, 2011·Uirusu·Teruo Sano
Jul 9, 2014·Annual Review of Microbiology·Ricardo FloresSantiago F Elena
Sep 26, 2001·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·R Flores
Mar 21, 2007·Archives of Virology·M EirasJ A Daròs
Apr 1, 2004·The Plant Journal : for Cell and Molecular Biology·Ulrike VogtMichael Wassenegger
Sep 1, 2007·Molecular Plant Pathology·Robert A Owens
Nov 1, 2006·Molecular Plant Pathology·Gustavo Gómez, Vicente Pallás
Sep 4, 2008·Cellular Microbiology·Efthimia Mina TsagrisKriton Kalantidis
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Biao Ding
Jan 16, 2010·Journal of Integrative Plant Biology·Ying Wang, Biao Ding
Jul 3, 2004·Trends in Plant Science·Martin Tabler, Mina Tsagris
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Natalia Kovalskaya, Rosemarie W Hammond
Apr 29, 2016·The Plant Journal : for Cell and Molecular Biology·Athanasios DalakourasMichael Wassenegger
Sep 1, 2009·Viruses·Robert A Owens, Rosemarie W Hammond
Sep 1, 2009·Viruses·Ricardo FloresJosé-Antonio Daròs
Feb 21, 2018·The Journal of General Virology·Taro Tsushima, Teruo Sano

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