PMID: 8589424Jan 1, 1996Paper

A single nucleotide substitution in the alpha a gene confers oat pathogenicity to barley stripe mosaic virus strain ND18

Molecular Plant-microbe Interactions : MPMI
J J Weiland, M C Edwards

Abstract

A 236-nucleotide region from the alpha a gene of strain CV42 (pathogenic to oat), when substituted for the homologous region in strain ND18 (nonpathogenic to oat), was shown previously to confer a near wild-type oat pathogenicity to this strain (Weiland and Edwards, 1994, Virology 201: 116-126). The data suggested that six amino acid substitutions in the alpha a gene were responsible for the differences in oat pathogenicity, and that threonine-724, encoded by CV42, might be a critical amino acid in determining pathogenicity of barley stripe mosaic virus (BSMV) to oat. In the present work, codons specifying T-724, I-764, and N-785 (encoded by CV42 RNA alpha) were substituted individually and in combination for those coding for P-724, T-764, and K-785 (encoded by ND18 RNA alpha), respectively, by site-directed mutagenesis. The core K-733, T-734, and K-736 positions (CV42) were substituted for Q-733, S-734, and Q-736 (ND18) as a single block. The results of inoculations with these mutants indicate that the C2261-->A2261 nucleotide substitution (P-724-->T-724) by itself is sufficient to enable strain ND18 to infect oat plants, although poorly. Additional substitution of CV42 codons into ND18 RNA alpha at the remaining five position...Continue Reading

Citations

Mar 1, 2001·Molecular Plant Pathology·D M Lawrence, A O Jackson
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G AtabekovN A Shapka
Jan 8, 2009·Plant Physiology·Steven R Scofield, Richard S Nelson
Apr 30, 2009·Annual Review of Phytopathology·Andrew O JacksonMi Yeon Lee
Feb 13, 2001·The Journal of General Virology·O FedorkinS Yu Morozov
Mar 10, 2019·Plant Molecular Biology·Ravi Kant, Indranil Dasgupta

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