A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient for proper chromosome segregation in Pseudomonas aeruginosa

PloS One
Paulina JeczGrazyna Jagura-Burdzy

Abstract

Among the mechanisms that control chromosome segregation in bacteria are highly-conserved partitioning systems comprising three components: ParA protein (a deviant Walker-type ATPase), ParB protein (a DNA-binding element) and multiple cis-acting palindromic centromere-like sequences, designated parS. Ten putative parS sites have been identified in the P. aeruginosa PAO1 genome, four localized in close proximity of oriC and six, diverged by more than one nucleotide from a perfect palindromic sequence, dispersed along the chromosome. Here, we constructed and analyzed P. aeruginosa mutants deprived of each single parS sequence and their different combinations. The analysis included evaluation of a set of phenotypic features, chromosome segregation, and ParB localization in the cells. It was found that ParB binds specifically to all ten parS sites, although with different affinities. The P. aeruginosa parS mutant with all ten parS sites modified (parSnull) is viable however it demonstrates the phenotype characteristic for parAnull or parBnull mutants: slightly slower growth rate, high frequency of anucleate cells, and defects in motility. The genomic position and sequence of parS determine its role in P. aeruginosa biology. It tran...Continue Reading

References

Mar 1, 1992·Molecular Microbiology·N Ogasawara, H Yoshikawa
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K MullisH Erlich
Jun 14, 1996·Journal of Molecular Biology·M Lobocka, M Yarmolinsky
Jun 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·O ResnekovR Losick
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·D C LinA D Grossman
Jan 23, 1999·Science·O RodionovM Yarmolinsky
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·M H Rashid, A Kornberg
Aug 10, 2000·Molecular Microbiology·K GerdesR Bugge Jensen
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y Yamaichi, H Niki
Mar 12, 2002·Molecular Microbiology·R EdgarM Yarmolinsky
Jun 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Patrick H ViollierLucy Shapiro
Dec 23, 2004·Molecular Microbiology·Michael A Fogel, Matthew K Waldor
Dec 24, 2004·The Journal of Biological Chemistry·Piotr JakimowiczMark J Buttner
May 18, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas A LeonardJan Löwe
Jul 21, 2006·Journal of Bacteriology·Djenann Saint-DicLyn Sue Kahng
Aug 24, 2006·Molecular Microbiology·Heath MurrayJeff Errington
Nov 10, 2006·The Journal of Biological Chemistry·Simon RinggaardKenn Gerdes
Dec 13, 2006·Genes & Development·Michael A Fogel, Matthew K Waldor
May 15, 2007·Journal of Bacteriology·Yoshiharu YamaichiMatthew K Waldor
Jul 20, 2007·Molecular Microbiology·Dagmara JakimowiczKeith F Chater

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Methods Mentioned

BETA
PCRs
PCR
electrophoretic mobility shift assay

Software Mentioned

Elements
AxioVision Rel .
Alpha View
NIS
AlphaView

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