PMID: 9425157Feb 14, 1998Paper

A single point mutation controls the cholesterol dependence of Semliki Forest virus entry and exit

The Journal of Cell Biology
M VashishthaMargaret Kielian

Abstract

Membrane fusion and budding are key steps in the life cycle of all enveloped viruses. Semliki Forest virus (SFV) is an enveloped alphavirus that requires cellular membrane cholesterol for both membrane fusion and efficient exit of progeny virus from infected cells. We selected an SFV mutant, srf-3, that was strikingly independent of cholesterol for growth. This phenotype was conferred by a single amino acid change in the E1 spike protein subunit, proline 226 to serine, that increased the cholesterol independence of both srf-3 fusion and exit. The srf-3 mutant emphasizes the relationship between the role of cholesterol in membrane fusion and virus exit, and most significantly, identifies a novel spike protein region involved in the virus cholesterol requirement.

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Citations

Sep 21, 2007·European Biophysics Journal : EBJ·Elodie Teissier, Eve-Isabelle Pécheur
Dec 17, 2005·Nature Reviews. Microbiology·Margaret Kielian, Félix A Rey
Oct 19, 2005·Gene Therapy·E PiverJ-C Pagès
Aug 4, 2001·AIDS Research and Human Retroviruses·Z LiaoJ E Hildreth
Jun 12, 2003·Current Opinion in Hematology·Alfredo Garzino-Demo, Robert C Gallo
Apr 17, 2009·Journal of Virology·Brian P HannahGary H Cohen
Jul 10, 2009·Journal of Virology·Amanda HaferRaquel Hernandez
May 3, 2013·Journal of Virology·Claudia Sánchez-San MartínMargaret Kielian
Aug 10, 2000·Journal of Virology·Y E Lu, M Kielian
Nov 20, 2002·Journal of Virology·Andrey V SamsonovFredric S Cohen
Mar 9, 2002·Journal of Virology·Anna AhnMargaret Kielian
Jun 28, 2003·Journal of Virology·Karin StiasnyFranz X Heinz
Feb 14, 2003·Journal of Virology·Xinyong ZhangMargaret Kielian
Mar 16, 2004·Journal of Virology·Don L GibbonsMargaret Kielian
May 28, 2005·Journal of Virology·Barry-Lee WaartsJan Wilschut
Jun 10, 2003·Microbiology and Molecular Biology Reviews : MMBR·Nathalie Chazal, Denis Gerlier
Apr 24, 2012·Journal of Biomedical Science·Szu-Cheng KuoSzecheng J Lo
May 17, 2006·PLoS Medicine·Isabelle SchuffeneckerSylvain Brisse
Feb 15, 2013·PLoS Neglected Tropical Diseases·Regina Ching Hua LeeJustin Jang Hann Chu
Dec 12, 2007·PLoS Pathogens·Konstantin A TsetsarkinStephen Higgs
Feb 12, 2014·PLoS Pathogens·Josiah PetersenKenneth Briley
Mar 8, 2008·Emerging Infectious Diseases·Marcus PanningChristian Drosten
Feb 9, 2012·Advances in Virology·Jason Yat-Sing LeungJustin Jang Hann Chu

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