A single point mutation in the V3 region affects protein kinase Calpha targeting and accumulation at cell-cell contacts

Molecular and Cellular Biology
Alice VallentinD Joubert

Abstract

Given the importance of intercellular adhesion for many regulatory processes, we have investigated the control of protein kinase Calpha (PKCalpha) targeting to the cell-cell contacts. We have previously shown that, upon treatment of the pituitary cell line GH3B6 with thyrotropin-releasing hormone (TRH) or phorbol 12-myristate 13-acetate (PMA), human PKCalpha (hPKCalpha) is selectively targeted to the cell-cell contacts (42). Here we show that the D294G mutation of hPKCalpha, previously identified in a subpopulation of human tumors, induces the loss of this selective targeting. The D294G mutant is instead targeted to the entire plasma membrane, including the cell-cell contacts, and the duration of the first rapid and transient translocation induced by TRH (42) is longer than that of the wild-type enzyme (93.3 versus 22.5 s), coinciding with the duration of the [Ca(2+)](i) increase. We found that in the presence or absence of PMA, RACK1 is never localized at the cell-cell contacts nor was it coimmunoprecipitated with hPKCalpha wild type or the D294G mutant. In contrast, PMA treatment or long-term TRH stimulation resulted in the presence of F-actin and beta-catenin at the cell-cell contacts and their exclusion from the rest of the...Continue Reading

References

Nov 1, 1992·Journal of Cellular Biochemistry·J Hagmann, M M Burger
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D Mochly-RosenJ Lopez
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A J LinnenbachH Koprowski
Oct 13, 1995·The Journal of Biological Chemistry·D RonD Mochly-Rosen
Nov 1, 1993·The Journal of Clinical Endocrinology and Metabolism·V AlvaroD Joubert
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·D RonD Mochly-Rosen
Nov 1, 1995·Molecular Biology of the Cell·P C Baciu, P F Goetinck
Jun 28, 1996·The Journal of Biological Chemistry·G C BlobeY A Hannun
Jan 17, 1997·The Journal of Biological Chemistry·M YedovitzkyR Nesher
Dec 1, 1995·Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research·C L JonesM A Kane
Sep 18, 1997·Trends in Neurosciences·F BattainiS Govoni
May 30, 1998·The Journal of Biological Chemistry·N C GuérineauP Mollard
Jun 6, 1998·The Journal of Biological Chemistry·A Horowitz, M Simons
Sep 25, 1998·The Journal of Biological Chemistry·A Horowitz, M Simons
Oct 3, 1998·The Journal of Biological Chemistry·R PrekerisD M Terrian
Sep 2, 1999·Molecular Reproduction and Development·C M Pauken, D G Capco
Sep 10, 1999·The Journal of Biological Chemistry·D RonA Gordon
Oct 6, 1999·Current Opinion in Cell Biology·M S Steinberg, P M McNutt
Dec 22, 1999·Current Opinion in Cell Biology·A E AplinR L Juliano

❮ Previous
Next ❯

Citations

Sep 23, 2003·Experimental Cell Research·Virginie GeeraertJean de Barry
May 12, 2009·The Journal of Biological Chemistry·Barthélémy DioufDominique Joubert
Mar 2, 2006·Molecular and Cellular Biology·Alejandra CollazosDominique Joubert
Nov 26, 2009·American Journal of Physiology. Endocrinology and Metabolism·Alexandra C Newton
Oct 17, 2008·Physiological Reviews·Susan F Steinberg
Oct 15, 2003·The Journal of Cell Biology·Masaki ShigetaKiyotoshi Sekiguchi
Nov 1, 2013·Medical Oncology·Julhash U KaziLars Rönnstrand
Dec 9, 2004·Immunology Letters·Alison M Michie, Rinako Nakagawa
Dec 3, 2014·Biochimica Et Biophysica Acta·J Martin-LiberalM Linch
Feb 12, 2019·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·David ScheieMorten Grauslund
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Rong MaSteven C Sansom
Aug 26, 2017·Biomolecular Concepts·Rishi Kant SinghArbind Acharya
Mar 8, 2018·Critical Reviews in Biochemistry and Molecular Biology·Alexandra C Newton
May 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Julia A CallenderAlexandra C Newton
Jun 20, 2018·Nature Communications·Shai RosenbergMarc Sanson
Dec 12, 2020·Advances in Biological Regulation·Adrian R Black, Jennifer D Black
Mar 5, 2003·Journal of Cell Science·Frédéric HollandeGraham S Baldwin
Nov 25, 2003·Journal of Cell Science·Corinne Quittau-PrévostelDominique Joubert
Mar 27, 2010·Biochimica Et Biophysica Acta·Nibedita MahataManoj K Chakrabarti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.