A single site for N-linked glycosylation in the envelope glycoprotein of feline immunodeficiency virus modulates the virus-receptor interaction.

Retrovirology
B J WillettM J Hosie

Abstract

Feline immunodeficiency virus (FIV) targets helper T cells by attachment of the envelope glycoprotein (Env) to CD134, a subsequent interaction with CXCR4 then facilitating the process of viral entry. As the CXCR4 binding site is not exposed until CD134-binding has occurred then the virus is protected from neutralising antibodies targeting the CXCR4-binding site on Env. Prototypic FIV vaccines based on the FL4 strain of FIV contain a cell culture-adapted strain of FIV Petaluma, a CD134-independent strain of FIV that interacts directly with CXCR4. In addition to a characteristic increase in charge in the V3 loop homologue of FIVFL4, we identified two mutations in potential sites for N-linked glycosylation in the region of FIV Env analogous to the V1-V2 region of HIV and SIV Env, T271I and N342Y. When these mutations were introduced into the primary GL8 and CPG41 strains of FIV, the T271I mutation was found to alter the nature of the virus-CD134 interaction; primary viruses carrying the T271I mutation no longer required determinants in cysteine-rich domain (CRD) 2 of CD134 for viral entry. The T271I mutation did not confer CD134-independent infection upon GL8 or CPG41, nor did it increase the affinity of the CXCR4 interaction, sug...Continue Reading

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Citations

Jan 20, 2010·Archives of Virology·Bruno Marques TeixeiraM J Hosie
Nov 10, 2011·Viruses·Margaret J HosieBrian J Willett
Dec 14, 2011·Viruses·Julia C Kenyon, Andrew M L Lever
Nov 10, 2009·Veterinary Immunology and Immunopathology·Martin KraaseMargaret J Hosie
Sep 3, 2013·Current Opinion in Virology·Brian J Willett, Margaret J Hosie
Aug 6, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Sueli Akemi TaniwakiJoão Pessoa Araujo
May 8, 2015·Journal of Virology·Birgit G Bradel-TrethewayHector C Aguilar

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Methods Mentioned

BETA
glycosylation
electrophoresis
transfection
flow cytometry

Software Mentioned

BLAST

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