A Single Spherical Assembly of Protein Amyloid Fibrils Formed by Laser Trapping

Angewandte Chemie
Ken-Ichi YuyamaHiroshi Masuhara

Abstract

Protein amyloids have received much attention owing to their correlation with serious diseases and to their promising mechanical and optical properties as future materials. Amyloid formation has been conducted by tuning temperature and chemical conditions, so that its nucleation and the following growth are analyzed as ensemble dynamics. A single spherical assembly of amyloid fibrils of cytochrome c domain-swapped dimer was successfully generated upon laser trapping. The amyloid fibrillar structure was confirmed by fluorescence characterization and electron microscopy. The prepared spheres were further manipulated individually in solution to fabricate a three-dimensional microstructure and a line pattern. Amyloid formation dynamics and amyloid-based microstructure fabrication are demonstrated based on direct observation of a single spherical assembly, which foresees a new approach in amyloid studies.

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Citations

Jul 26, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Tsung-Han LiuHiroshi Masuhara
Feb 23, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Mitsuhiro MatsumotoYasuyuki Tsuboi
Jun 5, 2021·ACS Applied Materials & Interfaces·Sayaka HashimotoYasuyuki Tsuboi
Oct 30, 2021·Chemical Communications : Chem Comm·Shun HirotaNaoya Kobayashi

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