A sisRNA/miRNA Axis Prevents Loss of Germline Stem Cells during Starvation in Drosophila

Stem Cell Reports
Ismail Osman, Jun Wei Pek

Abstract

Animal reproduction responds to nutritional status. During starvation, Drosophila and Caenorhabditis elegans enter a period of reproductive diapause with increase apoptosis, while maintaining a stable pool of germline stem cells (GSCs). How GSCs are protected is not understood. Here, we show that a sisRNA/miRNA axis maintains ovarian GSCs during starvation in Drosophila. Starvation induces the expression of an ovary-enriched sisRNA sisR-2, which negatively regulates GSC maintenance via a fatty acid metabolism gene dFAR1. sisR-2 promotes the expression of bantam, which in turn inhibits the activity of sisR-2, forming a negative feedback loop. Therefore, bantam acts as a buffer to counteract sisR-2 activity to prevent GSC loss during starvation. We propose that the sisR-2/bantam axis confers robustness to GSCs in Drosophila.

Citations

Nov 16, 2019·PLoS Genetics·Mandy Li-Ian Tay, Jun Wei Pek
Oct 24, 2020·Cell Death and Differentiation·Ismail Osman, Jun Wei Pek
Feb 20, 2020·Current Opinion in Insect Science·Kun-Yang Lin, Hwei-Jan Hsu
Apr 11, 2021·Methods : a Companion to Methods in Enzymology·Amanda Yunn Ee Ng, Jun Wei Pek
Nov 30, 2021·PLoS Genetics·Kelly VooJun Wei Pek

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Methods Mentioned

BETA
transgenic

Software Mentioned

modENCODE
BLAST
Adobe Photoshop
Vienna RNAfold
RNAhybrid

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