A slide latex agglutination test for the rapid detection of antibodies in serum against porcine parvovirus

Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health
J LüH Chen

Abstract

A slide latex agglutination test (LAT) was developed and evaluated to detect serum antibodies against porcine parvovirus. Porcine parvovirus antigen was obtained by 10% PEG-6000 and 0.5 mol/l sodium chloride precipitation, and inactivated by 0.1% methanal. Two per cent suspensions of latex particles (0.5-0.8 microm) were coated by adding an equal volume of porcine parvovirus antigen at 0.34 microg/ml. Repeatability of latex agglutination test was evaluated with a panel of 100 sera using the same and different antigen lots. A good agreement between LAT and haemagglutination inhibit assay was observed. Because of convenience and speed of performance, this method would be used widely in clinic examination.

References

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Citations

Jul 31, 2013·The Journal of Veterinary Medical Science·Quang Lam TruongTae-Wook Hahn
Jul 16, 2016·Mediators of Inflammation·Elisa GarciaAntonio Ibarra

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