PMID: 7021154Jun 1, 1981Paper

A small-angle X-ray scattering study of the complex formation between elongation factor Tu . GTP and valyl-tRNA Val I from Escherichia coli

European Journal of Biochemistry
R OsterbergP Elias

Abstract

The complex formation between elongation factor Tu (EF-Tu) . GTP and valyl-tRNA Val 1 has been investigated using the small-angle X-ray scattering titration technique. The main species observed is a 1:1 complex with a stability constant log K greater than or equal to 6. The corresponding interaction between EF-Tu . GTP and non-aminoacylated tRNA appears to be much weaker with an estimated log K approximately equal to 4. The radius of gyration determined for the EF-Tu . GTP -- valyl-tRNA Val 1 complex is larger (R = 3.6 nm) than that of EF-Tu . GTP (R = 2.5 nm). Likewise, the maximum distance within this complex is larger (Dmax = 12.5 nm) than the one within EF-Tu . GTP (Dmax = 8.5 nm). These data as well as the p(r) curve are consistent with a multiellipsoid model for the complex. From this model it is indicated that the acceptor stem of tRNA is attached to EF-Tu and that the anticodon stem and loop protrude into the solution.

References

Jul 24, 1978·Biochimica Et Biophysica Acta·B Sjöberg, P Elias
Jan 1, 1979·Annual Review of Biochemistry·T Blumenthal, G G Carmichael
Aug 16, 1974·Nature·J D RobertusA Klug
Mar 1, 1970·European Journal of Biochemistry·I PilzF Lynen
Jul 13, 1970·Biochemical and Biophysical Research Communications·K Ghosh, H P Ghosh
Jun 5, 1980·Journal of Molecular Biology·R G Knowlton, M Yarus
Jan 1, 1963·Progress in Biophysics and Molecular Biology·O KRATKY

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