A small aptamer with strong and specific recognition of the triphosphate of ATP

Journal of the American Chemical Society
Peter L SazaniJ W Szostak

Abstract

We report the in vitro selection of an RNA-based ATP aptamer with the ability to discriminate between adenosine ligands based on their 5' phosphorylation state. Previous selection of ATP aptamers yielded molecules that do not significantly discriminate between ligands at the 5' position. By applying a selective pressure that demands recognition of the 5' triphosphate, we obtained an aptamer that binds to ATP with a Kd of approximately 5 muM, and to AMP with a Kd of approximately 5.5 mM, a difference of 1100-fold. This aptamer demonstrates the ability of small RNAs to interact with negatively charged moieties.

References

Mar 11, 1994·Science·R D JenisonB Polisky
Aug 5, 1993·Nature·M Sassanfar, J W Szostak
Nov 14, 1998·Journal of Molecular Biology·F Huang, M Yarus
Jun 29, 2000·Annual Review of Biochemistry·D S Wilson, J W Szostak
Aug 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan H Davis, Jack W Szostak
Nov 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Wade C WinklerRonald R Breaker
Jan 30, 2003·RNA·Rob Knight, Michael Yarus
Dec 23, 2003·BMC Evolutionary Biology·Dayal SaranDonald H Burke

❮ Previous
Next ❯

Citations

May 7, 2011·Analytical and Bioanalytical Chemistry·Mayurbhai PatelHaidong Huang
Aug 2, 2012·Quarterly Reviews of Biophysics·Robert T Batey
Jun 2, 2009·Analytical Chemistry·Seung Soo OhH Tom Soh
Nov 8, 2007·Journal of the American Chemical Society·Liyun LinJohn C Chaput
Jun 6, 2013·Journal of the American Chemical Society·Yuri ImaizumiNaoki Sugimoto
Mar 19, 2011·The Journal of Organic Chemistry·Christoph KrönerClemens Richert
Sep 9, 2011·ACS Nano·Jolene L LauM G Finn
May 3, 2011·The Journal of Biological Chemistry·Achim BoltzBjoern Hock
Mar 10, 2012·Science·Jeremy S PaigeSamie R Jaffrey
Mar 21, 2009·Annual Review of Biochemistry·Adam Roth, Ronald R Breaker
Nov 23, 2006·BMC Bioinformatics·Venkata ThodimaYouping Deng
Sep 10, 2011·Journal of Nucleic Acids·Cindy MeyerAndrea Rentmeister
Nov 15, 2012·Journal of Nucleic Acids·Maureen McKeague, Maria C Derosa
Oct 25, 2012·Journal of Nucleic Acids·Yuuya Kasahara, Masayasu Kuwahara
May 18, 2011·Nucleic Acids Research·H Hans SalamancaJohn T Lis
Sep 9, 2008·The International Journal of Biochemistry & Cell Biology·Andrew D EllingtonAngel Syrett
Jan 24, 2008·Biochimica Et Biophysica Acta·Juana Ledia TrianaFlor Herrera
Jul 14, 2007·Biomolecular Engineering·Regina StoltenburgBeate Strehlitz
Feb 6, 2009·Journal of Molecular Recognition : JMR·Xing ZhangRichard C Willson
Aug 20, 2011·Wiley Interdisciplinary Reviews. RNA·Katarzyna Matylla-KulinskaRenée Schroeder
Jun 30, 2010·Cellular & Molecular Biology Letters·Teresa Janas, Tadeusz Janas
Jul 28, 2006·Chembiochem : a European Journal of Chemical Biology·Jonas NoeskeJens Wöhnert
Nov 1, 2011·Macromolecular Rapid Communications·Ali Rostami, Mark S Taylor
Jan 25, 2012·Chembiochem : a European Journal of Chemical Biology·Yuanfeng PangNorio Teramae
Jan 19, 2019·Chemical Communications : Chem Comm·Shun NakanoTakashi Morii
Apr 20, 2007·Chemistry & Biodiversity·Xi ChenAndrew D Ellington
May 17, 2007·Chemistry : a European Journal·Tao LiShaojun Dong
Nov 19, 2010·Chembiochem : a European Journal of Chemical Biology·Veli C OzalpLars F Olsen
Jan 30, 2007·The Analyst·Wei LiXiaohai Yang
Oct 25, 2011·Chembiochem : a European Journal of Chemical Biology·Hanyang YuJohn C Chaput
Aug 23, 2019·Journal of Molecular Evolution·Andrew PlebanekMark A Ditzler

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
equilibrium ultrafiltration

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.