PMID: 7521532Aug 30, 1994Paper

A small mitochondrial double-stranded (ds) RNA element associated with a hypovirulent strain of the chestnut blight fungus and ancestrally related to yeast cytoplasmic T and W dsRNAs

Proceedings of the National Academy of Sciences of the United States of America
J J Polashock, B I Hillman

Abstract

A small double-stranded (ds) RNA element was isolated from a moderately hypovirulent strain of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr. from eastern New Jersey. Virulence was somewhat lower in the dsRNA-containing strain than in a virulent dsRNA-free control strain, but colony morphology and sporulation levels were comparable. A library of cDNA clones was constructed, and overlapping clones representing the entire genome were sequenced. The 2728-bp dsRNA was considerably smaller than previously characterized C. parasitica dsRNAs, which are 12-13 kb and ancestrally related to the Potyviridae family of plant viruses. Sequence analysis revealed one large open reading frame, but only if mitochondrial codon usage (UGA = Trp) was invoked. Nuclease assays of purified mitochondria confirmed that the dsRNA was localized within mitochondria. Assuming mitochondrial translation, the deduced amino acid sequence had landmarks typical of RNA-dependent RNA polymerases. Alignments of the conserved regions indicate that this dsRNA is more closely related to yeast T and W dsRNAs and single-stranded RNA bacteriophages such as Q beta than to other hypovirulence-associated dsRNAs.

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Citations

Mar 11, 2004·Virus Research·Eugene V Makeyev, Jonathan M Grimes
Jul 1, 1997·Trends in Genetics : TIG·J R MarienfeldA Brennicke
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·C B Monteiro-VitorelloH Bertrand
Nov 22, 2013·Nucleic Acids Research·Matus ValachGertraud Burger
Feb 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rosa Esteban, Tsutomu Fujimura
Feb 18, 2014·Archives of Virology·Ryoko KitaharaTatsuhiro Ezawa
Jul 9, 2014·Annual Review of Phytopathology·Jiatao Xie, Daohong Jiang
Feb 21, 2016·Fungal Biology·Subha DasAndrew R Pitman
Jul 1, 2008·Journal of Theoretical Biology·Laura A Shackelton, Edward C Holmes
Sep 11, 2007·Medical Hypotheses·Ger P A Bongaerts, Lambert P van den Heuvel
Jun 10, 2003·Acta Microbiologica Et Immunologica Hungarica·J VargaC Vágvölgyi
Sep 16, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Corine N SchoebelDaniel Rigling

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