A small protein from the bop-brp intergenic region of Halobacterium salinarum contains a zinc finger motif and regulates bop and crtB1 transcription

Molecular Microbiology
Valery TarasovDieter Oesterhelt

Abstract

Bacteriorhodopsin, the photosynthetic protein of Halobacterium salinarum, is optimally expressed under anaerobic growth conditions. We identified Brz (OE3104F, bacteriorhodopsin-regulating zinc finger protein), a new regulator of the bop gene. It is a small protein with a zinc finger motif, encoded directly upstream of the bop gene in the same orientation. Deletion of the brz gene caused a large decrease of bop mRNA levels as shown by Northern blot and microarray analysis. A similar effect was obtained by site-directed mutagenesis of Cys and His residues in the zinc finger motif, indicating the importance of this motif for the function of the protein. In silico analysis of the genomes from H. salinarum and other archaea revealed a large family of similar small zinc finger motif proteins, some of which may also be involved in transcription regulation of their adjacent genes.

References

Jan 1, 1989·Canadian Journal of Microbiology·S W ClineW F Doolittle
Oct 1, 1985·Journal of Bacteriology·F A PfeiferM C Betlach
Mar 1, 1987·Journal of Bacteriology·S W Cline, W F Doolittle
Oct 25, 1984·Nucleic Acids Research·M BetlachF Pfeifer
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·F Gropp, M C Betlach
Jan 13, 2000·Molecular Microbiology·V Y TarasovO V Fedorov
Apr 12, 2000·Molecular Microbiology·M L Holmes, M L Dyall-Smith
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·W V NgS DasSarma
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·N S BaligaS DasSarma
Mar 27, 2001·Nucleic Acids Research·F DiehlJ D Hoheisel
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R DunnH G Khorana
Mar 9, 2005·Molecular Microbiology·Matthias K Koch, Dieter Oesterhelt
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·E F MongodinF Rodriguez-Valera
Dec 31, 2005·Nucleic Acids Research·Nicolas HuloChristian J A Sigrist
Mar 1, 2007·Journal of Proteome Research·Christian KleinDieter Oesterhelt
Oct 25, 2007·PloS One·Jens TwellmeyerDieter Oesterhelt

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Citations

May 22, 2008·Archives of Microbiology·J SoppaC Lange
Jul 2, 2008·Archives of Microbiology·Friedhelm PfeifferDieter Oesterhelt
Jan 8, 2010·Nature·Matthew A HumbardJulie A Maupin-Furlow
Feb 11, 2012·Nucleic Acids Research·Elizabeth G WilbanksMarc T Facciotti
Aug 7, 2012·Journal of Bacteriology·Priya DasSarmaShiladitya DasSarma
Apr 14, 2009·Journal of Bacteriology·Andy WendeDieter Oesterhelt
Aug 16, 2011·Journal of Bacteriology·Antoinette M DummerRonald F Peck
Nov 23, 2012·BMC Microbiology·Matthias SchlesnerDieter Oesterhelt
Mar 5, 2010·Molecular Microbiology·Katarina FurtwänglerDieter Oesterhelt
Mar 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Micaela GianiRosa María Martínez-Espinosa
Jul 5, 2017·BMC Genomics·Henk BolhuisFrancisco Rodriguez-Valera
Apr 12, 2020·Genes·Mike Dyall-SmithFriedhelm Pfeiffer
Jan 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Vanessa KrauspeWolfgang R Hess

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Prosite
GenePix Pro

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