Oct 24, 2018

A small RNA controls bacterial resistance to gentamicin during iron starvation

BioRxiv : the Preprint Server for Biology
Sylvia ChareyrePierre Mandin

Abstract

Phenotypic resistance describes a bacterial population that becomes transiently resistant to an antibiotic without requiring a genetic change. We here investigated the role of the small regulatory RNA (sRNA) RyhB, a key contributor to iron homeostasis, in the phenotypic resistance of Escherichia coli to various classes of antibiotics. We found that RyhB induces resistance to gentamicin, an aminoglycoside that targets the ribosome, when iron is scarce. RyhB induced resistance is due to the inhibition of respiratory complexes Nuo and Sdh activities. These complexes, which contain numerous Fe-S clusters, are crucial for generating a proton motive force (pmf) that allows gentamicin uptake. RyhB directly represses the expression of nuo and sdh operons by binding to their mRNAs, thereby inhibiting their translation. Indirectly, RyhB also inhibits the maturation of Nuo and Sdh by repressing synthesis of the Isc Fe-S biogenesis machinery. Notably, our study identifies nuo as a new direct RyhB target and shows that respiratory complexes activity levels are predictive of the bacterial sensitivity to gentamicin. Altogether, these results unveil a new role for RyhB in the adaptation to antibiotic stress, an unprecedented consequences of it...Continue Reading

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Mentioned in this Paper

Biological Adaptation to Stress
Small Nuclear RNA
Aminoglycoside [EPC]
MRNA Maturation
Proton Therapy
Gentamicins
Aminoglycosides
Antibiotic throat preparations
Complex (molecular entity)
General Adaptation Syndrome

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