A small RNA regulates the expression of genes involved in iron metabolism in Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
Eric Massé, Susan Gottesman

Abstract

A small RNA, RyhB, was found as part of a genomewide search for novel small RNAs in Escherichia coli. The RyhB 90-nt RNA down-regulates a set of iron-storage and iron-using proteins when iron is limiting; it is itself negatively regulated by the ferric uptake repressor protein, Fur (Ferric uptake regulator). RyhB RNA levels are inversely correlated with mRNA levels for the sdhCDAB operon, encoding succinate dehydrogenase, as well as five other genes previously shown to be positively regulated by Fur by an unknown mechanism. These include two other genes encoding enzymes in the tricarboxylic acid cycle, acnA and fumA, two ferritin genes, ftnA and bfr, and a gene for superoxide dismutase, sodB. Fur positive regulation of all these genes is fully reversed in an ryhB mutant. Our results explain the previously observed inability of fur mutants to grow on succinate. RyhB requires the RNA-binding protein, Hfq, for activity. Sequences within RyhB are complementary to regions within each of the target genes, suggesting that RyhB acts as an antisense RNA. In sdhCDAB, the complementary region is at the end of the first gene of the sdhCDAB operon; full-length sdhCDAB message disappears and a truncated message, equivalent in size to the reg...Continue Reading

References

Jun 3, 1988·Science·J A Imlay, S Linn
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R A LeaseM Belfort
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·N MajdalaniS Gottesman
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·O VytvytskaA von Gabain
Mar 9, 1999·Trends in Microbiology·K M WassarmanG Storz
Jul 27, 1999·Journal of Bacteriology·M ZhengG Storz
Oct 9, 1999·Journal of Bacteriology·L EscolarV de Lorenzo
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·E Hajnsdorf, P Régnier
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
Jul 13, 2000·Cell·K M Wassarman, G Storz
Oct 6, 2000·Annual Review of Microbiology·C Ratledge, L G Dover
Feb 27, 2001·Journal of Bacteriology·D D SledjeskiA Zhang
Mar 17, 2001·Molecular Microbiology·N MajdalaniS Gottesman
Apr 3, 2001·Current Opinion in Microbiology·K Hantke
Jul 11, 2001·Genes & Development·K M WassarmanS Gottesman
Jul 13, 2001·Current Biology : CB·L ArgamanS Altuvia
Jan 24, 2002·Molecular Cell·Aixia ZhangGisela Storz
Jan 24, 2002·Molecular Cell·Thorleif MøllerPoul Valentin-Hansen

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Citations

Oct 14, 2008·Genes & Nutrition·G CairoS Recalcati
Jun 19, 2010·Archives of Microbiology·Dominik SchillingUlrike Gerischer
Sep 14, 2007·Applied Microbiology and Biotechnology·Hua FuQi Jin
Apr 24, 2009·Applied Microbiology and Biotechnology·Rajkumar PylaYean-Sung Jung
Aug 10, 2010·Applied Microbiology and Biotechnology·Per KlemmViktoria Hancock
Aug 31, 2010·Applied Microbiology and Biotechnology·Rajkumar PylaYean-Sung Jung
May 25, 2011·Applied Microbiology and Biotechnology·Elisabeth Sonnleitner, Dieter Haas
Oct 3, 2008·Current Microbiology·Yean-Sung Jung, Young-Man Kwon
Oct 14, 2006·Molecular Genetics and Genomics : MGG·Jianhua YangMark R O'brian
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Shelley M PayneNicola M L Davies
Jun 24, 2010·The Journal of Microbiology·Shana MilesD Scott Merrell
Sep 3, 2013·Bioorganic & Medicinal Chemistry·Johannes MeitertBeatrix Suess
Apr 6, 2004·Current Opinion in Microbiology·Gisela StorzAixia Zhang
May 26, 2005·Trends in Genetics : TIG·Susan Gottesman
May 28, 2013·Methods : a Companion to Methods in Enzymology·M A Faner, A L Feig
Jun 26, 2003·Trends in Microbiology·Jörgen Johansson, Pascale Cossart
May 22, 2003·Microbes and Infection·Riny JanssenJaap T van Dissel
Dec 10, 2002·Current Opinion in Chemical Biology·Alexander HüttenhoferJean Pierre Bachellerie
May 7, 2003·Current Opinion in Microbiology·Eric MasséSusan Gottesman
Oct 1, 2009·Chemical Reviews·Zhen MaDavid P Giedroc
Dec 17, 2008·Nature Reviews. Microbiology·Kevin J Waldron, Nigel J Robinson
Nov 9, 2004·Nature Structural & Molecular Biology·Peter J MikuleckyAndrew L Feig
Jun 20, 2008·Molecular BioSystems·Jeffrey J TaborAndrew D Ellington
May 14, 2011·Metallomics : Integrated Biometal Science·Pierre CornelisTiffany Vinckx
Jan 24, 2013·Metallomics : Integrated Biometal Science·Amanda G Oglesby-Sherrouse, Erin R Murphy
Oct 2, 2002·Molecular Microbiology·Noel BaichooJohn D Helmann
Aug 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ahmed GaballaJohn D Helmann
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Namiko MitaraiKim Sneppen
Nov 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Todd M LinkRichard G Brennan
Dec 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Razika Hussein, Han N Lim
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jovanka T KooWyndham W Lathem
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sheetal R ModiJames J Collins
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hironori OtakaHiroji Aiba
Mar 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Liron ArgamanShoshy Altuvia
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marie-Pier CaronDaniel A Lafontaine
Jan 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·William H DePasMatthew R Chapman
Sep 10, 2010·The Journal of Biological Chemistry·Susan FischerAnita Marchfelder
Apr 22, 2008·The Journal of Biological Chemistry·Amanda G OglesbyMichael L Vasil

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