A Small Secreted Virulence-Related Protein Is Essential for the Necrotrophic Interactions of Sclerotinia sclerotiorum with Its Host Plants

PLoS Pathogens
Xueliang LyuJiasen Cheng

Abstract

Small, secreted proteins have been found to play crucial roles in interactions between biotrophic/hemi-biotrophic pathogens and plants. However, little is known about the roles of these proteins produced by broad host-range necrotrophic phytopathogens during infection. Here, we report that a cysteine-rich, small protein SsSSVP1 in the necrotrophic phytopathogen Sclerotinia sclerotiorum was experimentally confirmed to be a secreted protein, and the secretion of SsSSVP1 from hyphae was followed by internalization and cell-to-cell movement independent of a pathogen in host cells. SsSSVP1∆SP could induce significant plant cell death and targeted silencing of SsSSVP1 resulted in a significant reduction in virulence. Through yeast two-hybrid (Y2H), coimmunoprecipitation (co-IP) and bimolecular fluorescence complementation (BiFC) assays, we demonstrated that SsSSVP1∆SP interacted with QCR8, a subunit of the cytochrome b-c1 complex of mitochondrial respiratory chain in plants. Double site-directed mutagenesis of two cysteine residues (C38 and C44) in SsSSVP1∆SP had significant effects on its homo-dimer formation, SsSSVP1∆SP-QCR8 interaction and plant cell death induction, indicating that partial cysteine residues surely play crucial ro...Continue Reading

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Citations

Mar 14, 2017·Microbiological Research·Asma SafdarDaolong Dou
Mar 31, 2017·BMC Genomics·Shirin SeifbarghiDwayne D Hegedus
Jun 30, 2018·Annual Review of Phytopathology·Liangsheng XuWeidong Chen
Jul 27, 2018·Phytopathology·Xiaofei Liang, Jeffrey A Rollins
Jul 29, 2020·Molecular Plant-microbe Interactions : MPMI·Antonio Muñoz-BarriosAntonio Molina
Dec 9, 2017·Chemical Society Reviews·Fernanda Oliveira ChagasMônica Tallarico Pupo
Jun 4, 2019·Applied Microbiology and Biotechnology·Haizhen NieHongmei Zeng
Sep 27, 2020·Cellular Microbiology·Annika KönigBernhard Hube
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Dec 12, 2020·Frontiers in Plant Science·Toby E Newman, Mark C Derbyshire
Jun 19, 2018·Insect Biochemistry and Molecular Biology·Zhuhong WangMing-Shun Chen

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Methods Mentioned

BETA
electrophoresis
transgenic
reverse transcription PCR
two-hybrid
Y2H
co-immunoprecipitation
Co-IP
protein folding
PCR
immunoprecipitation

Software Mentioned

Jalview
COBALT
SignalP
DGE

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