A soft lithographic approach to fabricate patterned microfluidic channels

Analytical Chemistry
Ali KhademhosseiniRobert Langer

Abstract

The control of surface properties and spatial presentation of functional molecules within a microfluidic channel is important for the development of diagnostic assays and microreactors and for performing fundamental studies of cell biology and fluid mechanics. Here, we present a simple technique, applicable to many soft lithographic methods, to fabricate robust microchannels with precise control over the spatial properties of the substrate. In this approach, the patterned regions were protected from oxygen plasma by controlling the dimensions of the poly(dimethylsiloxane) (PDMS) stamp and by leaving the stamp in place during the plasma treatment process. The PDMS stamp was then removed, and the microfluidic mold was irreversibly bonded to the substrate. The approach was used to pattern a nonbiofouling poly(ethylene glycol)-based copolymer or the polysaccharide hyaluronic acid within microfluidic channels. These nonbiofouling patterns were then used to fabricate arrays of fibronectin and bovine serum albumin as well as mammalian cells. In addition, further control over the deposition of multiple proteins onto multiple or individual patterns was achieved using laminar flow. Also, cells that were patterned within channels remained...Continue Reading

References

Mar 18, 1999·Journal of Biomechanical Engineering·A FolchM Toner
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·S TakayamaG M Whitesides
Jun 29, 2001·Nature·S TakayamaG M Whitesides
Jul 12, 2001·Annual Review of Biomedical Engineering·G M WhitesidesD E Ingber
Jan 26, 2002·Science·Abraham D StroockGeorge M Whitesides
Sep 28, 2002·Analytical Chemistry·Wei ZhanRichard M Crooks
Jul 12, 2003·Science·Todd H RiderMark A Hollis
Sep 13, 2003·Science·Yukiko M YamashitaMargaret T Fuller

❮ Previous
Next ❯

Citations

Nov 13, 2008·Cytotechnology·Adrian T O'NeillGlenn M Walker
Jun 10, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sam H AuAaron R Wheeler
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ali KhademhosseiniJoseph P Vacanti
May 24, 2007·Tissue Engineering·Hyoungshin ParkOmid C Farokhzad
Feb 4, 2010·Tissue Engineering. Part B, Reviews·Nasim AnnabiFariba Dehghani
Dec 21, 2006·Journal of Biomaterials Science. Polymer Edition·Ali KhademhosseiniRobert Langer
May 3, 2014·Analytical Biochemistry·Min S WangNitin Nitin
Dec 11, 2013·Tissue Engineering. Part B, Reviews·Serge OstrovidovAli Khademhosseini
Jan 28, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·George Warren GreeneBrett Paull
Oct 6, 2009·Biomaterials·Lindsey K FiddesAaron R Wheeler
Jan 31, 2006·Biotechnology and Bioengineering·Jordan S MillerJennifer L West
Dec 7, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Robert GauvinAli Khademhosseini
Nov 6, 2012·Biotechnology Journal·Sasha Cai Lesher-PerezShuichi Takayama
Aug 4, 2015·ACS Applied Materials & Interfaces·Katharina KrumpholzKatrin Bui-Göbbels
Jul 3, 2015·Biotechnology Advances·Mario RothbauerPeter Ertl
Aug 1, 2015·Biomedical Microdevices·Nisarga NaikElliot L Chaikof
Jul 28, 2016·Scientific Reports·Ki-Hwan NamDeok-Ho Kim
Jun 28, 2008·Lab on a Chip·Sun Min KimKahp Yang Suh
Dec 20, 2008·Molecular Systems Biology·Zhizhong YinAndre Levchenko
Feb 27, 2009·Small·Masoud KhabiryAli Khademhosseini
May 4, 2007·Lab on a Chip·Yuk Kee CheungSamuel K Sia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.