A soft, stretchable and conductive biointerface for cell mechanobiology

Biomedical Microdevices
Irene BernardeschiLucia Beccai

Abstract

In mechanobiology the study of cell response to mechanical stimuli is fundamental, and the involved processes (i.e., mechanotransduction) need to be investigated by interfacing (mechanically and electrically) with the cells in dynamic and non-invasive natural-like conditions. In this work, we present a novel soft, stretchable and conductive biointerface that allows both cell mechanical stimulation and dynamic impedance recording. The biointerface stretchability and conductivity, jointly to the biocompatibility and transparency needed to perform cell culture studies, were obtained by exploiting the formation of wrinkles on the surface of a 90 nm thick conductive layer of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) on a pre-stretched 130 μm thick poly(dimethylsiloxane) (PDMS) substrate. Cell adhesion and proliferation of SH-SY5Y human neuroblastoma cells were evaluated, and cell differentiation on the corrugated surface was assessed. We demonstrate how the biointerface remains conductive when applying uniaxial strain up to 10%, and when cell culturing is performed. Finally, a reduction of about 30% of the relative impedance variation signal was measured, with respect to the control, as a result of the mec...Continue Reading

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Citations

May 18, 2017·Lab on a Chip·Xudong ZhangIoana Voiculescu
Jan 3, 2019·Advanced Materials·Laure V Kayser, Darren J Lipomi
Nov 15, 2018·Micromachines·Ioana VoiculescuFang Li
Oct 17, 2019·Lab on a Chip·Leonardo D GarmaFrancesca Santoro
Oct 4, 2020·Sensors·Thomas ChalklenSohini Kar-Narayan
Mar 2, 2021·Frontiers in Bioengineering and Biotechnology·Laura M FerrariJaume Del Valle
Apr 25, 2020·Nano-micro Letters·Yinlong TanWenjian Wu

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