PMID: 10556541Nov 11, 1999Paper

A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma

Journal of Immunological Methods
Hervé VollandJ Grassi

Abstract

We have developed a new enzyme immunometric assay for angiotensin II (AII) based on SPIE-IA technology (solid-phase immobilized epitope-immunoassay). A monoclonal antibody with optimal properties (mAb3 131) was selected from a series of 19 anti-AII mAbs. The mAb had to be purified from ascitic fluid in a specific manner in order to remove endogenous AII from the antibody-binding sites. We established a sensitive (minimum detectable concentration 0.5 pg/ml) and precise (CV below 15% in the 2-100 pg/ml range) SPIE-IA. Using different AII-related peptides, we observed that this new assay has a specificity profile that compares favourably with the corresponding competitive immunoassay. We have used the assay to measure AII in 42 plasma samples, and demonstrated a good correlation with values obtained using a commercial radioimmunoassay. Assay specificity was supported by HPLC fractionation experiments, confirming the absence of interference induced by endogenous AII-related products.

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Citations

Apr 25, 2014·Journal of Nutritional Science and Vitaminology·Akito MatsushimaMasao Hirayama
Oct 13, 2007·Analytical Biochemistry·Xiaoge QuSylvia Daunert
May 15, 2015·American Journal of Hypertension·Jan WysockiDaniel Batlle
Jul 4, 2012·Journal of Pharmaceutical and Biomedical Analysis·Nobuyo YoshidaHirokazu Matsumoto
Mar 15, 2015·Chemical Communications : Chem Comm·Christophe Créminon, Frédéric Taran
Mar 11, 2008·Early Human Development·Masakazu MiyawakiNorishige Yoshikawa
May 5, 2017·American Journal of Physiology. Renal Physiology·Jan WysockiMaryam Afkarian
Aug 1, 2006·The Journal of Clinical Investigation·Susan B GurleyThomas M Coffman
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sylvie GrégoireMartine Bruley Rosset
Aug 9, 2020·Oxidative Medicine and Cellular Longevity·Maha A AldubayanRehab M Elgharabawy
Jun 14, 2013·American Journal of Physiology. Renal Physiology·Jan WysockiDaniel Batlle
Dec 29, 2020·Small·Youngsun KimYuebing Zheng
Feb 26, 2010·Analytical Chemistry·Julia QuintonFrédéric Taran

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