PMID: 10556541Nov 11, 1999Paper

A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma

Journal of Immunological Methods
Hervé VollandJ Grassi


We have developed a new enzyme immunometric assay for angiotensin II (AII) based on SPIE-IA technology (solid-phase immobilized epitope-immunoassay). A monoclonal antibody with optimal properties (mAb3 131) was selected from a series of 19 anti-AII mAbs. The mAb had to be purified from ascitic fluid in a specific manner in order to remove endogenous AII from the antibody-binding sites. We established a sensitive (minimum detectable concentration 0.5 pg/ml) and precise (CV below 15% in the 2-100 pg/ml range) SPIE-IA. Using different AII-related peptides, we observed that this new assay has a specificity profile that compares favourably with the corresponding competitive immunoassay. We have used the assay to measure AII in 42 plasma samples, and demonstrated a good correlation with values obtained using a commercial radioimmunoassay. Assay specificity was supported by HPLC fractionation experiments, confirming the absence of interference induced by endogenous AII-related products.


Sep 27, 1979·Biochemical and Biophysical Research Communications·D RouxH L Cailla
Sep 15, 1976·European Journal of Biochemistry·J Massoulié, S Bon
Sep 1, 1992·Biologicals : Journal of the International Association of Biological Standardization·A R ter AvestC F van Kreyl
Mar 1, 1992·Chemical & Pharmaceutical Bulletin·F YasunagaM Takeyama
Apr 14, 1987·Biochemical and Biophysical Research Communications·L L McLaughlinP Pradelles
May 1, 1985·Hypertension·J NussbergerH R Brunner
Jan 1, 1987·Clinical and Experimental Hypertension. Part A, Theory and Practice·M KawamuraT Omae
Jan 1, 1982·Annual Review of Neuroscience·J Massoulié, S Bon
Nov 1, 1984·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·J NussbergerE Haber
Feb 10, 1995·Analytical Biochemistry·E EtienneP Pradelles
Sep 30, 1994·Journal of Immunological Methods·H VollandP Pradelles
Oct 30, 1996·Journal of Immunological Methods·E EtienneP Pradelles
Aug 29, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·F TaranP Pradelles
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE

❮ Previous
Next ❯


Apr 25, 2014·Journal of Nutritional Science and Vitaminology·Akito MatsushimaMasao Hirayama
Oct 13, 2007·Analytical Biochemistry·Xiaoge QuSylvia Daunert
May 15, 2015·American Journal of Hypertension·Jan WysockiDaniel Batlle
Jul 4, 2012·Journal of Pharmaceutical and Biomedical Analysis·Nobuyo YoshidaHirokazu Matsumoto
Mar 15, 2015·Chemical Communications : Chem Comm·Christophe Créminon, Frédéric Taran
Mar 11, 2008·Early Human Development·Masakazu MiyawakiNorishige Yoshikawa
May 5, 2017·American Journal of Physiology. Renal Physiology·Jan WysockiMaryam Afkarian
Aug 1, 2006·The Journal of Clinical Investigation·Susan B GurleyThomas M Coffman
Nov 8, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sylvie GrégoireMartine Bruley Rosset
Aug 9, 2020·Oxidative Medicine and Cellular Longevity·Maha A AldubayanRehab M Elgharabawy
Jun 14, 2013·American Journal of Physiology. Renal Physiology·Jan WysockiDaniel Batlle
Dec 29, 2020·Small·Youngsun KimYuebing Zheng
Feb 26, 2010·Analytical Chemistry·Julia QuintonFrédéric Taran

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.