PMID: 1198070Jan 1, 1975Paper

A solid-phase radioimmunoassay for C1q-binding immune complexes. I. Delta IgG as indicator molecule

Scandinavian Journal of Immunology
S E Svehag

Abstract

A solid-phase radioimmunoassay for detection of circulating C1q-binding immune complexes (IC) or heat-aggregated IgG (delta IgG) is described. Purified human C1q protein was adsorbed to a fixed area of polystyrene tube surface for 1 hr at 22 degrees C, pH 7.3, mu 0.15. Binding of delta IgG or IC to solid-phase C1q at 22 degrees C progressed over several hours and was enhanced at low mu (0.05). Heating of C1q (56 degrees C for 30 min) reduced the binding by 85%-90%. Binding of IC or delta IgG was retained after several weeks' storage of solid-phase C1q at 4 degrees C. Detection of 2-5 ng delta IgG and less than 50 ng IC (in antibody excess) was achieved in competitive binding or inhibition tests with [125I]delta IgG. Preliminary testing of 48 human sera indicated the usefulness of the assay for detection of IC in patient sera.

Citations

Jan 1, 1979·Scandinavian Journal of Immunology·A M Teppo, O Wager
Jan 1, 1976·Scandinavian Journal of Immunology·S AhlstedtC Wadsworth
Jan 1, 1980·Gastroenterologia Japonica·T ArimaH Nagashima
Oct 1, 1983·European Journal of Pediatrics·A Furuse, I Matsuda
Jan 1, 1980·CRC Critical Reviews in Clinical Laboratory Sciences·U E Nydegger, J S Davis
Nov 1, 1981·Annals of Clinical Biochemistry·D C KilpatrickW J Irvine
Jan 1, 1980·Journal of Immunological Methods·D A PohlS T Roodman
Feb 1, 1978·Immunochemistry·T Y Lin, D S Fletcher
Jan 1, 1981·Journal of Immunological Methods·D A PohlS T Roodman
Mar 26, 1982·Journal of Immunological Methods·B ZiolaK Penttinen
May 22, 1986·Journal of Immunological Methods·R Lahesmaa-RantalaA Toivanen
Mar 1, 1982·Journal of Clinical Microbiology·M M CollinsD P Stites
Dec 1, 1981·Kidney International·C A DavisC D West
Jul 1, 1981·La Ricerca in Clinica E in Laboratorio·P Cordiali Fei, P G Natali

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.