PMID: 6406611Jun 24, 1983Paper

A solid phase radioimmunoassay for detection of antibodies to extractable nuclear antigens

Journal of Immunological Methods
S Whittingham

Abstract

A solid phase radioimmunoassay is described for the detection of autoantibodies to the saline-soluble extractable nuclear antigens, ribonucleoprotein (RNP) and SS-B (or La). This assay depends on enrichment of antigens from a crude, commercially available (Pel Freez, U.S.A.) extract of rabbit thymus by absorption to the F(ab)2 fraction of specific high titre antibody attached to a microtitre plate. Serum antibody reactive with this antigen is then detected by 125I-labelled Protein A. The assay is simple and is more sensitive than the gel diffusion assays in general use for detecting such antibodies.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M R Lerner, J A Steitz
Jan 1, 1976·Journal of Immunological Methods·J W Goding
May 1, 1975·The Journal of Clinical Investigation·M A Alspaugh, E M Tan
Oct 1, 1967·The Journal of Experimental Medicine·D KofflerH G Kunkel
Apr 1, 1980·The Western Journal of Medicine·E M Tan

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Citations

Jun 26, 1987·Journal of Immunological Methods·T NishimakiR Kasukawa
Sep 20, 1991·Journal of Immunological Methods·H P SeeligM Renz

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