PMID: 2840453May 1, 1988Paper

A solid-phase reverse immunosorbent test for the detection of enterovirus IgM

Journal of Virological Methods
L MagniusH Norder


IgM antibodies against enterovirus antigen were determined by solid-phase reverse immunosorbent test (SPRIST). The 145 sera studied were sampled from cases of enterovirus infections diagnosed by virus isolation and/or complement fixation. In 91 sera from enterovirus infections diagnosed by virus isolation 11/40, 16/28 and 9/23 were positive in SPRIST against ECHO 3 and/or Coxsackie B3 antigen during the first, during the second and third, and after 3 weeks of illness, respectively. The corresponding figures for 85 sera from enterovirus infections diagnosed by a greater than or equal to 4-fold rise in complement fixing (CF) antibody titre against antigen from ECHO 18 and/or Coxsackie B5 antigen were 10/39, 12/20 and 9/26. None out of 22 sera with rheumatoid factor reacted in SPRIST, but 1/92 and 1/154 sera from blood donors was positive in SPRIST with Coxsackie B3 and ECHO 3 antigen, respectively. IgM antibodies against ECHO 3 antigen as determined by SPRIST were found to be cross-reactive over a broad range of enterovirus types and positive results were recorded with sera from infections with Coxsackie A9, B3, B4, B5, ECHO 3, 9, 17, 18, 25 and 30. Heterotypic titres in SPRIST were of the same magnitude (up to 25,600) as recorde...Continue Reading


Feb 1, 1986·Journal of Medical Virology·R C Lau
Feb 1, 1987·Journal of Virological Methods·A M van LoonJ T van der Logt
Jan 1, 1982·Journal of Medical Virology·J T van der LogtJ van der Veen
Jun 1, 1983·The Journal of Hygiene·J R Pattison
Jun 1, 1983·The Journal of Hygiene·P Morgan-Capner, C McSorley
Dec 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·M GOLDFIELDJ P FOX
Jul 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·L ROSEN, J KERN


Jan 1, 1993·Journal of Medical Virology·J A LiljeqvistS Jeansson
Nov 1, 1996·The Pediatric Infectious Disease Journal·Y XuA Linde
Oct 16, 2007·Journal of Medical Virology·Amal ElfaitouriJ Blomberg
Jan 1, 1990·Scandinavian Journal of Infectious Diseases·M GlimåkerP Olcén
Jan 1, 1993·Scandinavian Journal of Infectious Diseases·M GlimåkerM Forsgren
Jan 1, 1992·Scandinavian Journal of Infectious Diseases. Supplementum·M Glimåker
Jul 17, 1999·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·F CalabreseG Palù
Jan 1, 1991·Acta Neurologica Scandinavica·M RobergL Odkvist
Oct 1, 1995·Scandinavian Journal of Medicine & Science in Sports·G FrimanC Rolf
Mar 1, 1996·Clinical and Diagnostic Laboratory Immunology·T TolfvenstamK Broliden

Related Concepts

Related Feeds

Antibodies: Complement Activation

The complement system can be activated by antigen-associated antibody. In the classical pathway of complement activation, C1q, C4b, and C3b are all able to bind to the Fc portion of IgG or IgM. Find the latest research on antibodies and complement activation here.