PMID: 7522867Sep 1, 1994Paper

A solid-state 2H NMR investigation of purine motion in a 12 base pair RNA duplex

Journal of Magnetic Resonance. Series B
A C WangGary P Drobny

Abstract

Solid-state 2H NMR spectroscopy has been used to investigate the base dynamics of a RNA oligonucleotide with a defined sequence, [r(CGCGAAUUCGCG)]2, which contains the RNA analogue of the EcoRI binding site. The C8 protons of all purines in the self-complementary dodecamer were exchanged for deuterons. The quadrupole-echo lineshapes and spin-lattice relaxation times as a function of hydration for the sample in the form of the Na salt have previously been reported. In that study the 2H NMR lineshapes and T1 values of [r(CG*CG*A*A*UUCG*CG*)]2 were compared with those of the analogously labeled DNA sequence, [(CG*CG*A*A*TTCG*CG*)]2 (Wang et al., J. Am. Chem. Soc. 114, 6583, 1992). It was concluded that the amplitudes of purine motion for DNA and RNA are similar at all hydration levels; however, the rate difference observed at low-hydration levels may or may not persist at high hydration. Here the internal motions of the purine bases in the RNA oligomer have been thoroughly investigated. Three models were used to simulate the motion: (1) two-site jump, (2) diffusion in a cone, and (3) restricted diffusion on the surface of a cone. The purine motion is best simulated by the restricted-diffusion on a cone model with an amplitude of +...Continue Reading

Citations

Aug 12, 2009·Journal of Biomolecular NMR·Greg L OlsenGabriele Varani
Feb 1, 1995·Current Opinion in Biotechnology·F Aboul-ela, G Varani
Oct 1, 1996·Current Opinion in Structural Biology·L M McDowell, J Schaefer
Feb 16, 2008·Journal of the American Chemical Society·Greg L OlsenGary P Drobny
Dec 10, 2009·Journal of the American Chemical Society·Greg L OlsenGabriele Varani
Feb 16, 2010·The Journal of Physical Chemistry. B·Monica N Kinde-CarsonGary A Meints
Jun 24, 2020·Biochemical Society Transactions·Arun Kumar Sreemantula, Alexander Marchanka
Aug 29, 2017·Progress in Nuclear Magnetic Resonance Spectroscopy·Liliya Vugmeyster, Dmitry Ostrovsky
Sep 24, 2009·Journal of the American Chemical Society·Liliya VugmeysterRobert L Vold
May 21, 2008·Journal of the American Chemical Society·Gary A MeintsGary Drobny

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