PMID: 2116483Aug 1, 1990Paper

A soluble and immunoreactive fragment of pemphigus foliaceus antigen released by trypsinization of viable human epidermis

The Journal of Investigative Dermatology
C R MartinsLuis A Diaz

Abstract

Pemphigus foliaceus (PF) antigen is a transmembrane desmosomal glycoprotein (desmoglein I), part of which is located on the keratinocyte surface. Previous studies have shown that after trypsinization of viable human epidermis, this antigen is no longer detected on the surface of detached keratinocytes. It was not known, however, if this loss of antigenic activity was due to destruction, internalization, or cleavage of the antigen itself. In the present study we investigated the fate of the PF antigen after trypsinization of viable human skin. By using Concanavalin-A agarose affinity chromatography, we could partially purify an antigenic glycoprotein fraction that was released by trypsinization into the medium. This antigenic fraction was radiolabeled and tested by immunoprecipitation using sera from endemic pemphigus foliaceus or fogo selvagem (FS), non-endemic pemphigus foliaceus (NEPF), pemphigus vulgaris (PV), and bullous pemphigoid (BP) patients, and sera from normal subjects as controls. Immunoprecipitated labeled proteins were analyzed by SDS-PAGE and autoradiography. All FS sera (20 of 20 FS and five of five NEPF) and 46% of the PV sera (six of 13) immunoprecipitated a band of 45-kD molecular weight. Sera from FS patient...Continue Reading

Citations

May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·E Dabelsteen
Sep 27, 2003·Journal of the American Academy of Dermatology·Ana María Abrèu-VelezTakashi Hashimoto
Dec 1, 1992·The Journal of Investigative Dermatology·R S LabibG J Anhalt
Sep 1, 1992·The Journal of Investigative Dermatology·K RappersbergerJ R Stanley
Oct 27, 1997·The Journal of Investigative Dermatology·D ZillikensG J Giudice

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