A Solvent-Free Approach for Converting Cellulose Waste into Volatile Organic Compounds with Endophytic Fungi

Journal of Fungi
Tyler MaxwellJames K Harper

Abstract

Simple sugars produced from a solvent-free mechanocatalytic degradation of cellulose were evaluated for suitability as a growth medium carbon source for fungi that produce volatile organic compounds. An endophytic Hypoxylon sp. (CI-4) known to produce volatiles having potential value as fuels was initially evaluated. The growth was obtained on a medium containing the degraded cellulose as the sole carbon source, and the volatile compounds produced were largely the same as those produced from a conventional dextrose/starch diet. A second Hypoxylon sp. (BS15) was also characterized and shown to be phylogenetically divergent from any other named species. The degraded cellulose medium supported the growth of BS15, and approximately the same quantity of the volatile compounds was produced as from conventional diets. Although the major products from BS15 grown on the degraded cellulose were identical to those from dextrose, the minor products differed. Neither CI-4 or BS15 exhibited growth on cellulose that had not been degraded. The extraction of volatiles from the growth media was achieved using solid-phase extraction in order to reduce the solvent waste and more efficiently retain compounds having low vapor pressures. A comparison...Continue Reading

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Citations

Jun 25, 2020·Life·Erika Calla-QuispeAlfredo J Ibañez
Sep 10, 2021·Applied Microbiology and Biotechnology·Xi LiuJun-Hong Wang
Oct 26, 2021·Biotechnology and Applied Biochemistry·Nagarajan ArumugamPerarasu Thangavelu

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Datasets Mentioned

BETA
MH223406
MH465497
MH465498

Methods Mentioned

BETA
PCR

Software Mentioned

ITSx
MUSCLE
BLAST
BioPython
UNITE
Agilent MSD ChemStation
MEGA
GENEWIZ
Interactive Tree of Life ( iTOL )

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