A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy

Proceedings of SPIE
Xavier MichaletS Weiss

Abstract

We have recently developed a wide-field photon-counting detector having high-temporal and high-spatial resolutions and capable of high-throughput (the H33D detector). Its design is based on a 25 mm diameter multi-alkali photocathode producing one photo electron per detected photon, which are then multiplied up to 107times by a 3-microchannel plate stack. The resulting electron cloud is proximity focused on a cross delay line anode, which allows determining the incident photon position with high accuracy. The imaging and fluorescence lifetime measurement performances of the H33D detector installed on a standard epifluorescence microscope will be presented. We compare them to those of standard single-molecule detectors such as single-photon avalanche photodiode (SPAD) or electron-multiplying camera using model samples (fluorescent beads, quantum dots and live cells). Finally, we discuss the design and applications of future generation of H33D detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

Citations

Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·X MichaletS Cova
Feb 23, 2012·Journal of Biomedical Optics·Ryan A ColyerXavier Michalet

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