A spatiotemporally controllable chemical gradient generator via acoustically oscillating sharp-edge structures

Lab on a Chip
Po-Hsun HuangTony Jun Huang

Abstract

The ability to generate stable, spatiotemporally controllable concentration gradients is critical for resolving the dynamics of cellular response to a chemical microenvironment. Here we demonstrate an acoustofluidic gradient generator based on acoustically oscillating sharp-edge structures, which facilitates in a step-wise fashion the rapid mixing of fluids to generate tunable, dynamic chemical gradients. By controlling the driving voltage of a piezoelectric transducer, we demonstrated that the chemical gradient profiles can be conveniently altered (spatially controllable). By adjusting the actuation time of the piezoelectric transducer, moreover, we generated pulsatile chemical gradients (temporally controllable). With these two characteristics combined, we have developed a spatiotemporally controllable gradient generator. The applicability and biocompatibility of our acoustofluidic gradient generator are validated by demonstrating the migration of human dermal microvascular endothelial cells (HMVEC-d) in response to a generated vascular endothelial growth factor (VEGF) gradient, and by preserving the viability of HMVEC-d cells after long-term exposure to an acoustic field. Our device features advantages such as simple fabrica...Continue Reading

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Citations

Dec 17, 2015·Lab on a Chip·Toh G G AliciaNam-Trung Nguyen
May 10, 2016·Biomicrofluidics·Nitesh NamaFrancesco Costanzo
Jun 24, 2016·Lab on a Chip·Shwathy RamesanLeslie Y Yeo
Jul 29, 2016·Lab on a Chip·Murat KaynakTony Jun Huang
Apr 19, 2018·Lab on a Chip·Po-Hsun HuangTony Jun Huang
Feb 1, 2019·Lab on a Chip·Shuaiguo ZhaoTony Jun Huang
Nov 14, 2019·Lab on a Chip·Zeyu WangTony J Huang
Feb 28, 2020·Lab on a Chip·Hunter BachmanTony Jun Huang
May 1, 2020·Micromachines·Wasim RazaKwang-Yong Kim
Dec 2, 2018·Frontiers in Physiology·Francesco BasoliAlberto Rainer
Sep 12, 2019·Frontiers in Physiology·Adam RauffJeffrey A Weiss
Oct 21, 2017·Journal of Fluid Mechanics·Nitesh NamaFrancesco Costanzo
Oct 9, 2019·Advanced Science·Po-Hsun HuangTony Jun Huang
Apr 16, 2020·Advanced Biosystems·Alexander P HaringBlake N Johnson
Jan 6, 2018·Lab on a Chip·Hunter BachmanTony Jun Huang
Oct 8, 2020·Lab on a Chip·Jinsoo ParkHyung Jin Sung
Sep 3, 2018·Analytica Chimica Acta·Zeinab BagheriHamid Latifi
Oct 14, 2017·ACS Applied Materials & Interfaces·Xiaolong LuJoseph Wang

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