PMID: 6167290Aug 17, 1981Paper

A specific adenine nucleotide effect on the rat liver glucocorticoid receptor, demonstrated by aqueous two-phase partitioning

Biochimica Et Biophysica Acta
P A Andreasen

Abstract

The effect of mononucleotides on the cytosolic rat liver glucocorticoid receptor has been studied by the use of aqueous dextran-poly(ethylene glycol) two-phase partitioning. During incubations in 0.4 M KCl at 0 degrees C, millimolar concentrations of ADP and ATP, but not AMP, CTP, UTP and GTP, inhibit the increase in the receptor partition coefficient associated with receptor activation. This inhibition is counteracted by millimolar concentrations of theophylline and MgCl2. Two nonhydrolyzable analogues of ATP, alpha, beta-Methyleneadenosine 5'-triphosphate and beta, gamma-methyleneadenosine 5'-triphosphate, also inhibit the increase in the partition coefficient. alpha, beta-Methyleneadenosine 5'-triphosphate is much more potent than ATP in doing so, and this compound was also shown to reduce the amount of receptor to bind to DNA-Sepharose after the incubations. Thus, adenine nucleotides induce a change in the state of the receptor, apparently consisting in an inhibition of receptor activation.

Citations

Nov 1, 1982·Journal of Steroid Biochemistry·P A Andreasen
Nov 1, 1987·Journal of Steroid Biochemistry·E E Diehl, T J Schmidt
Apr 1, 1988·Endodontics & Dental Traumatology·T Uji, T Teramoto
Jun 1, 1989·Endodontics & Dental Traumatology·F M Andreasen
Sep 16, 2009·Psychological Medicine·C González-BlanchB Crespo-Facorro
Jan 1, 1995·Acta Psychiatrica Scandinavica. Supplementum·H J Möller
Jan 1, 1991·Critical Reviews in Food Science and Nutrition·J J MacheixA Fleuriet
Oct 31, 2015·The British Journal of Clinical Psychology·Emmeline Goodby, Andrew K MacLeod
May 25, 2007·European Psychiatry : the Journal of the Association of European Psychiatrists·Yves LecrubierRobert Morlock
May 26, 2007·European Psychiatry : the Journal of the Association of European Psychiatrists·Hans-Jürgen Möller
May 1, 1983·Molecular and Cellular Endocrinology·P A Andreasen
Jul 1, 1987·Molecular and Cellular Endocrinology·A M LeseneyJ J Befort

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