PMID: 8581323Jan 1, 1995Paper

A specific and sensitive method for the determination of linamarin in Urine

Natural Toxins
L CarlssonH Rosling

Abstract

A method is described for the quantitative determination of the cyanogenic glycoside linamarin. A preseparation procedure for urine samples was necessary to remove interfering substances. This was done by solid-phase extraction on a silica column containing cyclohexyl functional groups, which retained linamarin but allowed thiocyanate to pass unrestricted through the column. After elution of linamarin from the column by 35% (v/v) aqueous methanol, the glycoside was quantified following enzymatic hydrolysis, using the specified enzyme linamarase, and the free cyanide thus liberated was estimated spectrophotometrically. This method allowed quantification of linamarin in urine down to 10 mumol/l, with an estimated recovery of 91%. In 75 Tanzanian subjects consuming insufficiently processed cassava, the mean (+/- SD) urinary linamarin concentration was 104 (+/- 104) mumol/l (range 0 - 644 mumol/L), while that for thiocyanate was 486 (+/- 451) mumol/l (range 10-2,940 mumol/l), giving an approximate 1:5 molar concentration ratio between urinary linamarin and thiocyanate.

References

Apr 1, 1978·Journal of the Science of Food and Agriculture·R D Cooke
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Nov 19, 1982·Science·J H Cock
Jan 16, 1993·Lancet·H Rosling
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Citations

Jul 27, 1999·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L CarlssonH Rosling
May 1, 1998·Environmental Toxicology and Pharmacology·A J EssersJ H Koeman
Jun 30, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Benito Soto-Blanco, Silvana Lima Górniak
Mar 20, 2001·Public Health Nutrition·A OnaboluH Rosling
Dec 6, 2019·Metabolic Brain Disease·E Rivadeneyra-Domínguez, J F Rodríguez-Landa
Apr 11, 2019·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Tanja Schwerdtle

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