PMID: 8471054Apr 1, 1993Paper

A specific binding of the cholecystokinin-releasing peptide (monitor peptide) to isolated rat small-intestinal cells

The Biochemical Journal
R YamanishiE Sugimoto

Abstract

A specific binding of the cholecystokinin (CCK)-releasing peptide (monitor peptide) to isolated rat jejunal mucosal cells was investigated. The 125I-labelled purified monitor peptide bound to the rat jejunal cells, and a large excess amount of the non-labelled monitor peptide inhibited the binding. The binding was completed within 60 min at 37 degrees C. The optimum pH for the binding was 8-9. A Scatchard plot of the specific binding was linear, and the dissociation constant was 50 nM. The density of the monitor-peptide-binding sites was high in duodenum but low in ileal and absent in colonic mucosa. A recombinant monitor peptide and four kinds of point mutants of it were prepared. The binding of the mutant monitor peptides to the cells indicated that only a trypsin inhibitor of the mutants could bind to the mucosal cells. Human pancreatic secretory trypsin inhibitor inhibited the specific binding, but other trypsin inhibitors, i.e. bovine basic pancreatic trypsin inhibitor, soybean trypsin inhibitor, egg-white trypsin inhibitor, leupeptin, antipain and FOY-305, did not affect the specific binding at all. These findings suggested that the specific binding site for the monitor peptide on the jejunal mucosal cells has a trypsin-l...Continue Reading

Citations

Jan 1, 1997·Annual Review of Physiology·R A Liddle
Nov 14, 1997·The American Journal of Physiology·M JordinsonJ Calam
Jan 11, 2007·Bioscience, Biotechnology, and Biochemistry·Hirofumi HirayasuTohru Fushiki
Feb 7, 2001·Life Sciences·P LiW Y Chey
May 29, 2003·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·R GrafD Bimmler
Apr 25, 2006·Endocrinology and Metabolism Clinics of North America·Rolf Graf, Daniel Bimmler

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