PMID: 6976411Jan 1, 1982Paper

A specific biosynthetic marker for immature thymic lymphoblasts. Active synthesis of thymus-leukemia antigen restricted to proliferating cells

The Journal of Experimental Medicine
E V Rothenberg

Abstract

Large cortical thymocytes from C57BL/6-Tla(a) mice have been prepared rapidly and in high yield by a combination of centrifugal elutriation and differential binding to peanut agglutinin (PNA)-coated plates. The cells in these lymphoblast-rich fractions were clearly distinct from the majority of thymocytes, with up to 70 percent in the S or G(2) + M phases of the cell cycle and an average rate of [(35)S]methionine incorporation per cell up to 20 times higher than that of the majority population. The populations of cells resolved in this fractionation were characterized by monitoring their rates of synthesis of specific glycoproteins, thymus- leukemia antigen (TL) and the Lyt-2, Lyt-3 complex (Lyt-2/3), relative to their total protein synthesis. Cells that bound to PNA synthesized high levels of Lyt-2/3, consistent with their identification as cortical thymocytes. Those that failed to bind made little or no Lyt-2/3, as expected for medullary cells, The fraction of dividing lymphoblasts that bound to PNA was enriched in cortical thymocyte precursors, including all the large cells detectably active in synthesizing Lyt-2. It differed sharply from the small cortical cells, however, in the synthesis of TL. Although both populations di...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·E NakayamaL J Old
Jan 1, 1977·Immunological Reviews·H Cantor, E A Boyse
Jun 1, 1977·European Journal of Immunology·A M KruisbeekJ J Zijlstra
Oct 1, 1979·The Journal of Experimental Medicine·E Rothenberg, E A Boyse
Dec 1, 1979·European Journal of Immunology·P KisielowM Wysocka
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Jan 1, 1975·Cellular Immunology·C G FathmanI L Weissman
Jun 21, 1974·Science·H A Crissman, R A Tobey
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Feb 1, 1980·European Journal of Immunology·E L Larsson, A Coutinho
Jan 15, 1981·Nature·B J MathiesonU Hämmerling
Jan 1, 1980·Journal of Supramolecular Structure·E Rothenberg, D Triglia
Jan 1, 1981·Journal of Immunological Methods·M L MeistrichE S Richie

❮ Previous
Next ❯

Citations

Jun 1, 1991·Journal of Neuroscience Research·A RossiS Biagioni
Jan 1, 1989·Immunogenetics·K A BrorsonL Hood
Nov 1, 1985·Developmental Biology·E Rothenberg, J P Lugo
Jan 1, 1983·Human Immunology·P G LerchC Terhorst
Jul 1, 1988·Human Immunology·S ParkE J Yunis
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P M Cardarelli, M D Pierschbacher
Jan 1, 1983·The Journal of Experimental Medicine·E Rothenberg, D Triglia
Dec 1, 1986·The Journal of Experimental Medicine·D McKinnon, R Ceredig
Oct 1, 1987·The Journal of Experimental Medicine·C HannumJ Kappler
Aug 1, 1988·Immunological Reviews·E V RothenbergP D Boyer
Feb 1, 1992·Clinical and Experimental Immunology·S IkemotoM Maekawa
Jun 14, 2018·Glycobiology·Shigeko Kijimoto-OchiaiToshiaki Koda

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.