A specific competitive protein binding assay for serum 24,25-dihydroxyvitamin D in normal children and patients with nephrotic syndrome

Clinica Chimica Acta; International Journal of Clinical Chemistry
T ShimotsujiH Yabuuchi

Abstract

A specific competitive protein binding assay for 24,25-dihydroxyvitamin D by Sephadex LH-20 column chromatography, followed by high pressure liquid chromatography with normal rat kidney cytosol as the binding protein, was developed. The mean concentrations of serum 24,25-dihydroxyvitamin D of the cord, in newborn infants and in infants under 12 months of age were 0.90 +/- 0.40 (S.D.) ng/ml, 0.52 +/- 0.21 (S.D.) ng/ml and 1.20 +/- 0.38 (S.D.) ng/ml, respectively. These concentrations were significantly lower than those in children aged 1-15 years (1.96 +/- 0.83 (S.D.) ng/ml). The serum levels in the acute stage of the nephrotic syndrome were significantly reduced, and they increased in remission. These results show that patients wioth nephrotic syndrome have low levels of serum 24,25-dihydroxyvitamin D. This is probably due to its loss in the urine.

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Citations

Feb 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J CunninghamH L Makin
Jan 1, 1988·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M IshidaY Seino
Jan 1, 1991·Journal of Inherited Metabolic Disease·I YokotaY Kuroda
Mar 1, 1982·Calcified Tissue International·Y SeinoT Onishi
May 1, 1982·Calcified Tissue International·Y SeinoL V Avioli
Dec 1, 1984·Kidney International·K OlgaardE Slatopolsky
Jul 1, 1983·Acta paediatrica Scandinavica·T SakanoT Usui

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