A specific form of thioredoxin h occurs in plant mitochondria and regulates the alternative oxidase

Proceedings of the National Academy of Sciences of the United States of America
Eric GelhayeJean-Pierre Jacquot

Abstract

The plant mitochondrial thioredoxin (Trx) system has been described as containing an NADPH-dependent Trx reductase and Trx o. In addition to the mitochondrial isoform, Trx o, plants are known to contain several chloroplastic Trx isoforms and the cytosolic Trx h isoforms. We report here the presence in plant mitochondria of a Trx isoform (PtTrxh2) belonging to the Trx h group. Western blot analyses with mitochondrial proteins isolated from both poplar and GFP fusion constructs indicate that PtTrxh2 is targeted to plant mitochondria. The recombinant protein, PtTrxh2, has been shown to be reduced efficiently by the mitochondrial Trx reductase AtNTRA. PtTrxh2 is also able to reduce alternative oxidase homodimers and to allow its activation by pyruvate. In contrast, neither PtTrxh2 nor AtTrxo1 exhibits activity with several poplar glutathione peroxidases and especially a putative mitochondrial isoform. Incubation of PtTrxh2 with glutathione disulfide led to the formation of glutathionylated Trx, identified by mass spectrometry. The formation of a glutathione adduct increases the redox potential of PtTrxh2 from -290 to -225 mV. In addition to Trx o, this study shows that Trx h could also be present in mitochondria. This previously un...Continue Reading

References

May 15, 1991·Archives of Biochemistry and Biophysics·F MarcusB B Buchanan
Nov 1, 1996·Plant Molecular Biology·J Shi, M K Bhattacharyya
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·D P MaxwellL McIntosh
Feb 12, 2000·Biochimica Et Biophysica Acta·S D LemaireJ P Jacquot
May 10, 2001·Biochimica Et Biophysica Acta·O S WangensteenJ López Gorgé
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·C LaloiY Meyer
Mar 20, 2002·Methods in Enzymology·Yves MeyerJean Philippe Reichheld
Mar 21, 2002·Physiologia Plantarum·Eric GelhayeJean-Pierre Jacquot
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Roberta A Gottlieb, David J Granville
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Simona CasagrandePietro Ghezzi
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Miguel A Rodriguez MillaJ Perry Gustafson
Dec 17, 2003·FEBS Letters·Eric GelhayeJean Pierre Jacquot
Feb 21, 2004·Plant Physiology·Nicolas RouhierJean-Pierre Jacquot
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Yves BalmerBob B Buchanan
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. Schurmann, J.-P. Jacquot
May 4, 2004·Plant Physiology and Biochemistry : PPB·Eric GelhayeJean-Pierre Jacquot
Oct 26, 2005·Photosynthesis Research·Bob B BuchananJean-Pierre Jacquot

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Citations

Dec 7, 2010·Plant Cell Reports·Takushi Hachiya, Ko Noguchi
Feb 14, 2012·Plant Molecular Biology·Bornali GohainSudripta Das
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ling MengBob B Buchanan
Oct 23, 2009·The Journal of Biological Chemistry·Mariette BedhommeStéphane D Lemaire
Nov 30, 2011·Molecular & Cellular Proteomics : MCP·Mirko ZaffagniniStéphane D Lemaire
Nov 8, 2011·Antioxidants & Redox Signaling·Mirko ZaffagniniStéphane D Lemaire
Apr 26, 2012·Antioxidants & Redox Signaling·Yves MeyerChristophe Riondet
Dec 14, 2012·Antioxidants & Redox Signaling·Markus Schwarzländer, Iris Finkemeier
Jul 11, 2008·Plant Physiology·Fatima AlkhalfiouiFrançoise Montrichard
Aug 21, 2009·Annual Review of Genetics·Yves MeyerJean-Philippe Reichheld
May 3, 2005·Annual Review of Plant Biology·Bob B Buchanan, Yves Balmer
May 1, 2008·Annual Review of Plant Biology·Nicolas RouhierJean-Pierre Jacquot
Nov 11, 2011·International Journal of Molecular Sciences·Els KeunenAnn Cuypers
Oct 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Etienne H MeyerGéraldine Bonnard
Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laure MicheletStéphane D Lemaire
Jun 25, 2014·Antioxidants & Redox Signaling·Peter Geigenberger, Alisdair R Fernie
Feb 22, 2011·Annual Review of Plant Biology·A Harvey MillarDavid A Day
Jun 1, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Moêz SmiriEzzedine El Ferjani
Feb 28, 2013·Plant & Cell Physiology·Keisuke YoshidaToru Hisabori
Feb 16, 2016·Biochimica Et Biophysica Acta·Per HägglundBirte Svensson
Mar 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ian Max Møller
Dec 28, 2010·Journal of Plant Physiology·Joost T van DongenAlisdair R Fernie
Sep 21, 2010·Biochimica Et Biophysica Acta·Sang Gon KimSung-Kun Kim
Jan 13, 2009·Trends in Biochemical Sciences·Isabella Dalle-DonneAldo Milzani
Jan 13, 2009·Journal of Proteomics·Françoise MontrichardBob B Buchanan
Jul 16, 2008·Biochimica Et Biophysica Acta·Christopher Horst LilligArne Holmgren
Apr 29, 2008·Biochimica Et Biophysica Acta·Marie-Luise OelzeKarl-Josef Dietz
Jan 22, 2008·Biochimica Et Biophysica Acta·Nicolas RouhierJean-Pierre Jacquot
Nov 21, 2007·Mitochondrion·Ko Noguchi, Keisuke Yoshida

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