A specific G(o) heterotrimer couples somatostatin receptors to voltage-gated calcium channels in RINm5F cells

FEBS Letters
V E DegtiarF Kalkbrenner

Abstract

The peptide hormone somatostatin inhibits glucose-induced insulin secretion in the rat insulinoma RINm5F cells by inhibition of voltage-gated calcium channels. Here we used micro-injection of antisense oligonucleotides directed against subtypes of G-protein subunits to determine the subunit composition involved in somatostatin-induced inhibition of voltage-gated calcium channels in RINmF5 cells. Injection of antisense oligonucleotides annealing to the respective mRNA of G alpha o2, G beta 1 and G gamma 3 reduced the somatostatin-induced inhibition of calcium channels in these cells. Injection of antisense oligonucleotides directed against other G-protein subunits did not, suggesting that in RINm5F cells the somatostatin receptor couples to a G protein of G alpha o2 beta 1 gamma 3 composition. By using a selective agonist of type 2 somatostatin receptors (SSTR 2) NC 8-12, we identified this receptor as the subtype coupling to calcium channels in RINm5F cells.

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Citations

Mar 8, 2002·Cellular Signalling·Paul R Albert, Liliane Robillard
Mar 11, 2000·British Journal of Pharmacology·C PetrucciP Bagnoli
Apr 1, 1997·The European Journal of Neuroscience·C StrübingA M Wobus
Aug 18, 2004·Molecular and Cellular Biology·William F SchwindingerJanet D Robishaw
Sep 28, 2012·Zhurnal evoliutsionnoĭ biokhimii i fiziologii·A O Shpakov
May 16, 2001·General and Comparative Endocrinology·S G DedosM Takamiya
May 11, 2001·Neuropeptides·Z Csaba, P Dournaud
Mar 3, 1998·The Journal of Physiology·A C Dolphin
Aug 28, 2007·Pharmacology & Therapeutics·Davide Cervia, Paola Bagnoli
Aug 15, 2006·Cellular Immunology·Alexander DubeykovskiyJanet D Robishaw
Feb 3, 2019·The Journal of Biological Chemistry·Zack ZurawskiHeidi E Hamm
Mar 10, 2015·Molecular Endocrinology·Antonella MaffeiPaul E Harris
Dec 6, 2003·Pharmacological Reviews·Annette C Dolphin
Aug 1, 1997·Biochemical Pharmacology·J D Hildebrandt
Sep 26, 2003·Biochimica Et Biophysica Acta·Lars Neisig MøllerJens Juul Holst

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