PMID: 4893886Aug 1, 1969Paper

A specific inactivator of mammalian C'4 isolated from nurse shark (Ginglymostoma cirratum) serum

The Journal of Experimental Medicine
J A Jensen

Abstract

A material which specifically inactivates mammalian C'4 was isolated from low ionic strength precipitates of nurse shark serum. The C'4 inactivator was not detected in whole serum. The conditions of its generation and its immunoelectrophoretic behavior seem to indicate that it is an enzymatically formed cleavage product of a precursor contained in whole shark serum. The inactivator was partially purified and characterized. It had an S-value of 3.3 (sucrose gradient) which was in agreement with its retardation on gel filtration, was stable between pH 5.0 and 10.0, had a half-life of 5 min at 56 degrees C, pH 7.5, was inactivated by trypsin and was nontoxic. Its powerful anticomplementary activity in vitro and in vivo was solely due to the rapid inactivation of C'4; no other complement components were affected. No cofactor requirement was observed for the equally rapid inactivation of highly purified human and guinea pig C'4. The kinetics of C'4 inactivation and TAME hydrolysis, the greater anodic mobility of inactivated human C'4, and the influence of temperature on the rate of inactivation suggest that the inactivator is an enzyme and C'4 its substrate. This conclusion was supported by the more recent detection of a split produ...Continue Reading

References

May 1, 1968·The Journal of Surgical Research·B S LinnG B Snyder
Aug 1, 1957·The Journal of Experimental Medicine·O D RATNOFF, I H LEPOW

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Citations

Nov 1, 1995·Journal of Clinical Immunology·M M Frank
Jan 1, 1974·Medical Microbiology and Immunology·G Füst, G Fóris
Feb 1, 1974·Immunochemistry·L FullerJ A Jensen
Mar 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·C A DanielsA L Notkins
Jun 1, 1973·British Journal of Haematology·G SirchiaS Ferrone
Jan 23, 1999·Immunological Reviews·S L Smith
Oct 1, 1972·Journal of Periodontology·L ShapiroR E Stallard
Mar 1, 1972·Circulation Research·J A JensenL Franklin
Dec 7, 2007·Bioorganicheskaia khimiia·L V KozlovA P Kaplun
Apr 7, 1971·Nature: New Biology·M L SchulkindL W Clem
Jan 1, 1980·Microbiology and Immunology·H NagaiA Koda
Jan 1, 1973·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·C HammerW Brendel
Aug 1, 1971·Infection and Immunity·R T ActonE E Evans
Oct 1, 1971·Infection and Immunity·M C GarcesE Iglesias

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