PMID: 1209690Nov 11, 1975Paper

A specific, non-chromatographic radioimmunoassay for human plasma cortisol

Steroids
R J DashG D Niswender

Abstract

A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3-carboxymethyl-oxime conjugated to bovine serum albumin, had a high affinity (KA = 1.8 X 10(9) 1/mole) and capacity (2.3 X 10(-6) moles/L undiluted serum) for cortisol. The minimum detectable amount determined at the lower 95% confidence limit of the buffer control tubes was 8.3 +/- 4.7 pg/tube and a log dose - logit response standard curve was linear between 20 pg and 20 ng/tube. The antiserum was highly specific for cortisol with only corticosterone, cortisone, 11-deoxycortisol and 21-deoxycortisol showing significant cross-reaction (12.4, 6.6, 3.8 and 3.7%, respectively). The cross-reaction for the other tested naturally occurring and synthetic steroids did not exceed 1%. Regression analysis of cortisol concentration estimates obtained on 20 samples before and after Sephadex LH-20 column chromatography gave a coefficient of correlation (r) of 0.995 and a regression coefficient (b) of 1.04. Recovery of cortisol added to plasma samples was quantitative. The intra-assay error was 8.5% and the inter-assay error a...Continue Reading

References

Aug 1, 1972·The Journal of Clinical Endocrinology and Metabolism·H J RuderM B Lipsett
Feb 1, 1973·The Journal of Clinical Endocrinology and Metabolism·L de LacerdaC J Migeon
Jan 1, 1974·The Journal of Clinical Endocrinology and Metabolism·B G EnglandA R Midgley
Jun 1, 1972·Computers and Biomedical Research, an International Journal·W G DuddlesonG D Niswender
Jan 1, 1972·The Journal of Clinical Endocrinology and Metabolism·A A IsmailA R Midgley
Mar 1, 1963·The Journal of Clinical Endocrinology and Metabolism·B P MURPHYC J PATTEE

❮ Previous
Next ❯

Citations

Jan 1, 1991·European Journal of Applied Physiology and Occupational Physiology·T RönnemaaV A Koivisto
May 29, 2007·Indian Journal of Pediatrics·P S Sandesh KiranPrahbhjot Malhi
May 16, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Seth, L M Brown
Jul 15, 1986·Clinica Chimica Acta; International Journal of Clinical Chemistry·M G McConway, R S Chapman
Mar 1, 1976·Journal of Immunological Methods·S Comoglio, F Celada
Nov 12, 2013·Biosensors & Bioelectronics·Ajeet KaushikShekhar Bhansali
Mar 24, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Folke HammarqvistJan Wernerman
Jan 23, 1999·Burns : Journal of the International Society for Burn Injuries·K P SinghR J Dash
Nov 1, 1996·Psychoneuroendocrinology·J H Thakore, T G Dinan
Aug 26, 1998·Clinical Endocrinology·L V ScottT G Dinan
Dec 14, 1999·Clinical Endocrinology·L V ScottT G Dinan
Jul 23, 1981·The New England Journal of Medicine·M D GrossJ Z Villareal
May 1, 1994·Clinical Endocrinology·J H Thakore, T G Dinan
May 1, 1993·Clinical Physiology·J WernermanE Vinars
Dec 1, 1996·Acta Psychiatrica Scandinavica·J M Cooney, T G Dinan
Jan 1, 1997·Acta Psychiatrica Scandinavica·T G DinanC Berti
Oct 1, 1985·The Journal of Clinical Investigation·Y ShenkerR J Grekin
Mar 1, 1995·Anesthesia and Analgesia·J Van HemelrijckW Heyns
Apr 1, 1994·Journal of Basic and Clinical Physiology and Pharmacology·J H ThakoreT G Dinan
Feb 1, 1987·Journal of Neurosurgery·W F ChandlerG Ibarra-Perez
Apr 1, 1996·Journal of Endocrinological Investigation·R TanakolF Berker
Jan 1, 1993·The British Journal of Nutrition·M A McNurlanJ Wernerman
Jun 5, 1995·American Journal of Medical Genetics·E HeikkiläP Santavuori
Aug 1, 1982·Analytical Biochemistry·C R Gebauer, G A Rechnitz
Sep 1, 1988·Metabolism: Clinical and Experimental·K SammalkorpiM R Taskinen
Jan 1, 1979·Advances in Experimental Medicine and Biology·D E SchteingartH S Tsao
Mar 1, 1986·The American Journal of Cardiology·A P RocchiniJ Anderson
Feb 1, 1992·Scandinavian Journal of Clinical and Laboratory Investigation·B DuguéR Gräsbeck
Jan 1, 1989·Journal of Internal Medicine·K Sammalkorpi
Dec 1, 1983·Clinical Endocrinology·N FrisinaM Buemi
May 1, 1982·Clinical Endocrinology·T C GniadekJ Z Villareal
Apr 1, 1980·Clinical Endocrinology·R D HoeldtkeM G Nicholls
Jan 1, 1996·Journal of Basic and Clinical Physiology and Pharmacology·T G Dinan, L V Scott

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.