A specific pathway inducing autophagic cell death is marked by an IP3R mutation

Autophagy
David Lam, Pierre Golstein

Abstract

Three main advantages make Dictyostelium a very favorable model to study the induction of autophagic cell death in vitro. First, its small, sequenced and haploid genome facilitates genetic approaches. Second, the Dictyostelium genome does not encode the two main molecular families involved in apoptosis (caspases and bcl-2 family), which therefore cannot interfere in this case with autophagic cell death. Third, induction of autophagic cell death follows in this case a two-step process, namely starvation-induced sensitization leading to autophagy but not to death, followed by a DIF-1-induced pathway leading to cell death proper. The latter, DIF-1-induced pathway is defined experimentally, through sequential additions, and most important also genetically, through random mutagenesis leading in particular to the preparation and study of an iplA mutant. The iplA gene encodes the IP3 Receptor, and its mutation leads to the absence of vacuolization and of death when autophagic cell death is triggered. Further study of the DIF-1 pathway should shed additional light on the induction of autophagic cell death (as opposed to that of just autophagy) in Dictyostelium and by extension perhaps in other organisms.

Citations

Jan 10, 2009·Biochimica Et Biophysica Acta·Corinne GiustiPierre Golstein
Jul 17, 2009·PloS One·Renée GuérinLuis A Rokeach
Nov 18, 2008·Nature Reviews. Microbiology·Ralph R IsbergMatthew Heidtman
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Can M Ünala, Michael Steinert
Dec 2, 2008·Nature Reviews. Drug Discovery·Inki KimJohn C Reed
Mar 28, 2009·Cell Death and Differentiation·J M VicencioG Kroemer
Feb 15, 2011·Carcinogenesis·Mathias T Rosenfeldt, Kevin M Ryan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis